Human beta-Defensin 2 ELISA Kit | DEFB4A elisa kit
beta-Defensin 2 ELISA
Gene Names
DEFB4A; BD-2; SAP1; DEFB2; DEFB4; HBD-2; DEFB-2; DEFB102
Reactivity
Human
Synonyms
beta-Defensin 2; beta-Defensin 2 ELISA; DEFB4A elisa kit
Reactivity
Human
Sequence Length
64
Samples
Stool
Intra-assay Precision
The reproducibility was calculated by measuring each of two samples 20 times by one technician within the same assay.
Inter-assay Precision
The total reproducibility was independently measured by several different technicians on 3 different days.
Preparation and Storage
2 - 8 degree C.
Related Product Information for DEFB4A elisa kit
Intended Uses: This assay is intended for the determination of beta-defensin 2 in stool. For research use only,
Principle of the Assay: The 13-defensin 2 in standards and samples is bound to an available excess of polyclonal antibodies against 13-defensin 2, which are immobilized on the surface of the microtiter plate. After a washing step, to remove all interfering substances, the determination of bound 13-defensin 2 is carried out by adding a polyclonal anti 13- defensin 2 antibody, which is horseradish peroxidase labeled. After a washing step to remove the unbound components, the peroxidase substrate tetramethylbenzidine is added. Finally, the enzymatic reaction is terminated by an acidic stop solution. The color changes from blue to yellow and the absorbance is measured in the photometer at 450 nm. The intensity of the yellow color proportional to the 13- defensin 2 concentration in the sample. A dose response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standards.
Background/Introduction: The beta-defensins are an integral part of the congenital immune system and contribute with their antimicrobial effect to the barrier function of intestinal epithelial cells. Defensins exert a variable degree of antimicrobial activity against bacteria, fungi, and some enveloped viruses. Vertebrate defensins are classified as alpha- or betadefensins, based on their pattern of disulfide bridges. Nine human defensins of epithelial origin have been found, three of them being beta-defensins (HBD-1, -2 and -3). The expression of beta-defensins is induced by the pro-inflammatory cytokines and also through microorganisms (e.g. F. coli, H. pylori or P. aeruginosa). A beta-defensin-2 deficiency can, for example, be observed in the intestinal mucous of subjects with Crohn's disease. The defense system of the mucous membrane is therefore restricted and allows an increased invasion of bacteria, which could possibly lead to a typical infection in Crohn's disease subjects. Whether the beta-defensin-2 deficiency could even play a role in the development of Crohn's disease is currently being researched. As is the possibility that it is the probiotic bacterium, which produces beta-defensin.
Principle of the Assay: The 13-defensin 2 in standards and samples is bound to an available excess of polyclonal antibodies against 13-defensin 2, which are immobilized on the surface of the microtiter plate. After a washing step, to remove all interfering substances, the determination of bound 13-defensin 2 is carried out by adding a polyclonal anti 13- defensin 2 antibody, which is horseradish peroxidase labeled. After a washing step to remove the unbound components, the peroxidase substrate tetramethylbenzidine is added. Finally, the enzymatic reaction is terminated by an acidic stop solution. The color changes from blue to yellow and the absorbance is measured in the photometer at 450 nm. The intensity of the yellow color proportional to the 13- defensin 2 concentration in the sample. A dose response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standards.
Background/Introduction: The beta-defensins are an integral part of the congenital immune system and contribute with their antimicrobial effect to the barrier function of intestinal epithelial cells. Defensins exert a variable degree of antimicrobial activity against bacteria, fungi, and some enveloped viruses. Vertebrate defensins are classified as alpha- or betadefensins, based on their pattern of disulfide bridges. Nine human defensins of epithelial origin have been found, three of them being beta-defensins (HBD-1, -2 and -3). The expression of beta-defensins is induced by the pro-inflammatory cytokines and also through microorganisms (e.g. F. coli, H. pylori or P. aeruginosa). A beta-defensin-2 deficiency can, for example, be observed in the intestinal mucous of subjects with Crohn's disease. The defense system of the mucous membrane is therefore restricted and allows an increased invasion of bacteria, which could possibly lead to a typical infection in Crohn's disease subjects. Whether the beta-defensin-2 deficiency could even play a role in the development of Crohn's disease is currently being researched. As is the possibility that it is the probiotic bacterium, which produces beta-defensin.
Product Categories/Family for DEFB4A elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
7,038 Da
NCBI Official Full Name
beta-defensin 2
NCBI Official Synonym Full Names
defensin, beta 4A
NCBI Official Symbol
DEFB4A
NCBI Official Synonym Symbols
BD-2; SAP1; DEFB2; DEFB4; HBD-2; DEFB-2; DEFB102
NCBI Protein Information
beta-defensin 4A; defensin, beta 2; defensin, beta 4; skin-antimicrobial peptide 1
UniProt Protein Name
Beta-defensin 4A
Protein Family
UniProt Gene Name
DEFB4A
UniProt Synonym Gene Names
DEFB102; DEFB2; DEFB4; BD-2; hBD-2; SAP1
UniProt Entry Name
DFB4A_HUMAN
NCBI Description
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 4, an antibiotic peptide which is locally regulated by inflammation. [provided by RefSeq, Jul 2008]
Uniprot Description
defensin, beta 2: Has antibacterial activity (Potential). Belongs to the beta-defensin family. LAP/TAP subfamily.
Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 8p23.1
Cellular Component: Golgi lumen; extracellular region
Biological Process: G-protein coupled receptor protein signaling pathway; defense response to bacterium; innate immune response; immune response; chemotaxis
Research Articles on DEFB4A
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Product Notes
The Human DEFB4A defb4a (Catalog #AAA495092) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA495092 ELISA Kit recognizes Human DEFB4A. It is sometimes possible for the material contained within the vial of "beta-Defensin 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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