Typical Testing Data/Standard Curve (for reference only)
Human B lymphocyte antigen CD20 (MS4A1) ELISA Kit | MS4A1 elisa kit
Human B lymphocyte antigen CD20 (MS4A1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: MR-proANP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MR-proANP antibody and an MR-proANP-HRP conjugate. The assay sample and buffer are incubated together with MR-proANP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MR-proANP concentration since MR-proANP from samples and MR-proANP-HRP conjugate compete for the anti-MR-proANP antibody binding site. Since the number of sites is limited, as more sites are occupied by MR-proANP from the sample, fewer sites are left to bind MR-proANP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MR-proANP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]
Uniprot Description
MS4A1: This protein may be involved in the regulation of B-cell activation and proliferation. Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the MS4A family.
Protein type: Membrane protein, integral; Cell surface; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 11q12
Cellular Component: extracellular space; integral to plasma membrane; external side of plasma membrane
Molecular Function: epidermal growth factor receptor binding
Biological Process: B cell proliferation; humoral immune response
Disease: Immunodeficiency, Common Variable, 5
Research Articles on MS4A1
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Product Notes
The Human MS4A1 ms4a1 (Catalog #AAA7210091) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7210091 ELISA Kit recognizes Human MS4A1. It is sometimes possible for the material contained within the vial of "B lymphocyte antigen CD20 (MS4A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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