Human B cell receptor associated protein 31 (BCAP31) ELISA Kit | BCAP31 elisa kit

Human B cell receptor associated protein 31 (BCAP31) ELISA Kit

Cat. Num. AAA7205960

• Gene Names: BCAP31; CDM; DDCH; BAP31; 6C6-AG; DXS1357E
• Reactivity: Human
• Synonyms: B cell receptor associated protein 31 (BCAP31); Human B cell receptor associated protein 31 (BCAP31) ELISA Kit; BCAP31 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of BCAP31. No significant cross-reactivity or interference between BCAP31 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BCAP31 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BCAP31 elisa kit

Intended Uses: This BCAP31 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BCAP31. This ELISA kit for research use only!

Principle of the Assay: BCAP31 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BCAP31 antibody and an BCAP31-HRP conjugate. The assay sample and buffer are incubated together with BCAP31-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCAP31 concentration since BCAP31 from samples and BCAP31-HRP conjugate compete for the anti-BCAP31 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCAP31 from the sample, fewer sites are left to bind BCAP31-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCAP31 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for BCAP31 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 1705725
• NCBI GeneID: 10134
• Molecular Weight: 34,752 Da
• NCBI Official Full Name: B-cell receptor-associated protein 31
• NCBI Official Synonym Full Names: B-cell receptor-associated protein 31
• NCBI Official Symbol: BCAP31
• NCBI Official Synonym Symbols: CDM; DDCH; BAP31; 6C6-AG; DXS1357E
• NCBI Protein Information: B-cell receptor-associated protein 31; p28 Bap31; BCR-associated protein Bap31; 6C6-AG tumor-associated antigen
• UniProt Protein Name: B-cell receptor-associated protein 31
• Protein Family: B-cell receptor-associated protein
• UniProt Gene Name: BCAP31
• UniProt Synonym Gene Names: BAP31; DXS1357E; BCR-associated protein 31; Bap31
• UniProt Entry Name: BAP31_HUMAN

NCBI Description

This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16. [provided by RefSeq, Jan 2012]

Uniprot Description

BCAP31 iso2: May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis. Homodimer and heterodimer with BCAP29. Binds CASP8 (isoform 9) as a complex containing BCAP31, BCAP29, BCL2 and/or BCL2L1. Interacts with VAMP3, VAMP1 and membrane IgD immunoglobulins. May interact with ACTG1 and non-muscle myosin II. Interacts with PTPLB. Ubiquitous. Belongs to the BCAP29/BCAP31 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; membrane; clathrin-coated vesicle; endoplasmic reticulum; integral to plasma membrane; lipid particle; cytosol

Molecular Function: protein binding; MHC class I protein binding; protein complex binding

Biological Process: intracellular protein transport; ER to Golgi vesicle-mediated transport; antigen processing and presentation of peptide antigen via MHC class I; elevation of cytosolic calcium ion concentration; apoptosis; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of caspase activity; spermatogenesis; cell structure disassembly during apoptosis; elevation of mitochondrial calcium ion concentration

Disease: Deafness, Dystonia, And Cerebral Hypomyelination