Human ATG7 autophagy related 7 homolog (S. cerevisiae) ELISA Kit | ATG7 elisa kit

Human Ubiquitin-like modifier-activating enzyme ATG7, ATG7 ELISA Kit

Cat. Num. AAA906789

• Gene Names: ATG7; GSA7; APG7L; APG7-LIKE
• Reactivity: Human
• Synonyms: ATG7 autophagy related 7 homolog (S. cerevisiae); Human Ubiquitin-like modifier-activating enzyme ATG7; ATG7 ELISA Kit; Human Ubiquitin-like modifier-activating enzyme ATG7 (ATG7) ELISA kit; APG7-LIKE; APG7L; DKFZp434N0735; GSA7; APG7 autophagy 7-like; ubiquitin activating enzyme E1-like protein; ATG7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human ATG7. No significant cross-reactivity or interference between human ATG7 and analogues was observed.
• Sequence Length: 676

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 28.12 pg/ml -1800 pg/ml.
• Sensitivity: The minimum detectable dose of human ATG7 is typically less than 7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATG7 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ATG7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ATG7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATG7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATG7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 222144227
• NCBI GeneID: 10533
• NCBI Accession #: NP_001129503.2
• NCBI GenBank Nucleotide #: NM_001136031.2
• UniProt Accession #: O95352; Q7L8L0; Q9BWP2; Q9UFH4; B4E170; E9PB95
• Molecular Weight: 77,960 Da
• NCBI Official Full Name: ubiquitin-like modifier-activating enzyme ATG7 isoform b
• NCBI Official Synonym Full Names: autophagy related 7
• NCBI Official Symbol: ATG7
• NCBI Official Synonym Symbols: GSA7; APG7L; APG7-LIKE
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme ATG7; hAGP7; autophagy-related protein 7; ATG12-activating enzyme E1 ATG7; ATG7 autophagy related 7 homolog; ubiquitin activating enzyme E1-like protein; ubiquitin-activating enzyme E1-like protein
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme ATG7
• Protein Family: Ubiquitin-like modifier-activating enzyme
• UniProt Gene Name: ATG7
• UniProt Synonym Gene Names: APG7L; APG7-like; hAGP7
• UniProt Entry Name: ATG7_HUMAN

NCBI Description

This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq, Jan 2009]

Uniprot Description

ATG7: Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3. Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. Belongs to the ATG7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Autophagy

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: cytoplasm; axoneme; cytosol

Molecular Function: protein binding; protein homodimerization activity; APG8 activating enzyme activity; APG12 activating enzyme activity; ubiquitin activating enzyme activity; transcription factor binding

Biological Process: positive regulation of apoptosis; regulation of protein ubiquitination; protein ubiquitination; pyramidal neuron development; adult walking behavior; post-embryonic development; protein transport; cardiac muscle cell development; protein amino acid lipidation; protein catabolic process; positive regulation of autophagy; amino acid metabolic process; central nervous system neuron axonogenesis; cerebellar Purkinje cell layer development; protein modification process; C-terminal protein lipidation; liver development; cellular response to starvation; neurological system process; cellular response to nitrogen starvation; protein modification by small protein conjugation; positive regulation of protein modification process; positive regulation of protein catabolic process; mitochondrion degradation; cerebral cortex development; positive regulation of macroautophagy; negative regulation of apoptosis