Typical Testing Data/Standard Curve (for reference only)
Human Asc Type Amino Acid Transporter 1 (ASC1) ELISA Kit | ASC1 elisa kit
Human Asc Type Amino Acid Transporter 1 (ASC1) ELISA Kit
Gene Names
SLC7A10; ASC1; asc-1; HASC-1
Reactivity
Human
Synonyms
Asc Type Amino Acid Transporter 1 (ASC1); Human Asc Type Amino Acid Transporter 1 (ASC1) ELISA Kit; SLC7A10; Solute Carrier Family 7 Member 10; Neutral Amino Acid Transporter; y+ System; ASC1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of ASC1. No significant cross-reactivity or interference between ASC1 and analogues was observed.
Sequence Length
523
Samples
Human tissue homogenates, cell lysates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
< 0.055ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ASC1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ASC1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ASC1 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ASC1 in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ASC1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ASC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ASC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ASC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ASC1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ASC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ASC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ASC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for ASC1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,798 Da
NCBI Official Full Name
asc-type amino acid transporter 1
NCBI Official Synonym Full Names
solute carrier family 7 (neutral amino acid transporter light chain, asc system), member 10
NCBI Official Symbol
SLC7A10
NCBI Official Synonym Symbols
ASC1; asc-1; HASC-1
NCBI Protein Information
asc-type amino acid transporter 1
UniProt Protein Name
Asc-type amino acid transporter 1
UniProt Gene Name
SLC7A10
UniProt Synonym Gene Names
ASC1; Asc-1
UniProt Entry Name
AAA1_HUMAN
NCBI Description
SLC7A10, in association with 4F2HC (SLC3A2; MIM 158070), mediates high-affinity transport of D-serine and several other neutral amino acids (Nakauchi et al., 2000 [PubMed 10863037]).[supplied by OMIM, Mar 2008]
Uniprot Description
SLC7A10: Sodium-independent, high affinity transport of small neutral D- and L-amino acids. May play a role in the modulation of glutamatergic transmission through mobilization of D-serine at the glutamatergic synapse. Belongs to the amino acid-polyamine-organocation (APC) superfamily.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, SLC family
Chromosomal Location of Human Ortholog: 19q13.1
Cellular Component: membrane; integral to plasma membrane; plasma membrane
Molecular Function: L-serine transmembrane transporter activity; neutral amino acid transmembrane transporter activity
Biological Process: D-alanine transport; neutral amino acid transport; amino acid transport; ion transport; blood coagulation; D-serine transport; leukocyte migration; transmembrane transport; L-serine transport
Research Articles on ASC1
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Product Notes
The Human ASC1 slc7a10 (Catalog #AAA2023552) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2023552 ELISA Kit recognizes Human ASC1. It is sometimes possible for the material contained within the vial of "Asc Type Amino Acid Transporter 1 (ASC1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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