Human APP ELISA Kit | APP elisa kit

Human APP PicoKine ELISA Kit

Cat. Num. AAA175851

• Gene Names: APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
• Reactivity: Human
• Synonyms: APP; Human APP PicoKine ELISA Kit; Amyloid beta A4 protein; amyloid beta (A4) precursor protein; A4 amyloid protein; A4; A4_HUMAN; AAA; ABETA; ABPP; AD 1; AD1; AICD-50; AICD-57; AICD-59; AID(50); AID(57); AID(59); Alzheimer disease 1; Alzheimer disease amyloid protein; Alzheimer's disease amyloid protein; Amyloid beta (A4) precursor protein; Amyloid beta A4 protein precursor isoform b; Amyloid beta A4 protein precursor isoform c; Amyloid beta A4 protein precursor isoform a; Amyloid beta protein; Amyloid beta-peptide; Amyloid intracellular domain 50; Amyloid intracellular domain 57; Amyloid intracellular domain 59; Amyloid of aging and alzheimer disease; APP I; APPI; Beta amyloid peptide; Beta-APP40; Beta-APP42; C31; Cerebral vascular amyloid peptide; CTFgamma; CVAP; Gamma-CTF(50); Gamma-CTF(57); Gamma-CTF(59); Human mRNA for amyloid A4 precursor of Alzheimer's disease; peptidase nexin II; PN 2; PN II; PN-II; PN2; PreA4; Protease nexin II; Protease nexin-II; S-APP-alpha; S-APP-beta; APP elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human APP
• Sequence Length: 770

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 312pg/ml-20,000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human APP PicoKine ELISA Kit standard curve )

Related Product Information for APP elisa kit

Principle of the assay: human APP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for APP has been precoated onto 96-well plates. Standards(NSO,L18-L688) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for APP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human APP amount of sample captured in plate.
Background: Amyloid precursor protein (APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. Its primary function is not known, though it has been implicated as a regulator of synapse formation, neural plasticity and iron export. APP is best known and most commonly studied as the precursor molecule whose proteolysis generates beta amyloid (Abeta), a 39- to 42-amino acid peptide whose amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer's disease patients. APP undergoes posttranslational proteolytic processing by alpha-, beta-, and gamma-secretases. Alpha-secretase generates soluble amyloid protein, while beta- and gamma-secretases generate APP components with amyloidogenic features. These 2 processing pathways are mutually exclusive.

NCBI and Uniprot Product Information

• NCBI GI #: 112927
• NCBI GeneID: 351
• UniProt Accession #: P05067; P09000; P78438; Q13764; Q13778; Q13793; Q16011; B2R5V1; B4DII8; D3DSD1; D3DSD2; D3DSD3
• Molecular Weight: 84,521 Da
• NCBI Official Full Name: Amyloid beta A4 protein
• NCBI Official Synonym Full Names: amyloid beta (A4) precursor protein
• NCBI Official Symbol: APP
• NCBI Official Synonym Symbols: AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
• NCBI Protein Information: amyloid beta A4 protein; preA4; protease nexin-II; peptidase nexin-II; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); alzheimer disease amyloid protein; cerebral vascular amyloid peptide
• UniProt Protein Name: Amyloid beta A4 protein
• Protein Family: Amyloid beta A4 protein
• UniProt Gene Name: APP
• UniProt Synonym Gene Names: A4; AD1; APP; CVAP; PN-II; S-APP-alpha; S-APP-beta; AICD-59; AID(59); AICD-57; AID(57); AICD-50; AID(50)
• UniProt Entry Name: A4_HUMAN

NCBI Description

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014]

Uniprot Description

APP: a cell surface receptor that influences neurite growth, neuronal adhesion and axonogenesis. Cleaved by secretases to form a number of peptides, some of which bind to the acetyltransferase complex Fe65/TIP60 to promote transcriptional activation. The Abeta peptide is released from the cell, its extracellular deposition and accumulation form the main components of amyloid plaques in Alzheimer's disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis. Can promote transcription activation through binding to Fe65-Tip60 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(O) alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. Induces a RAGE-dependent pathway that activates p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB family members, the APBA family, JIP1, SHC1 and, NUMB and DAB1. Binding to DAB1 inhibits its serine phosphorylation. Associates with microtubules in the presence of ATP and in a kinesin-dependent manner. Amyloid beta-42 binds nAChRA7 in hippocampal neurons. Beta-amyloid associates with HADH2. Soluble APP binds, via its N-terminal head, to FBLN1. Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex- opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra- striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. 10 isoforms of the human protein are produced by alternative splicing. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non- neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Appican is expressed in astrocytes. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Belongs to the APP family.

Protein type: Receptor, misc.; Transcription factor; Apoptosis; Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 21q21.3

Cellular Component: Golgi apparatus; extracellular space; cell surface; integral to plasma membrane; integral to membrane; coated pit; intercellular junction; cytosol; ER to Golgi transport vesicle; lipid raft; ciliary rootlet; perinuclear region of cytoplasm; nuclear envelope lumen; cytoplasm; synapse; dendritic shaft; neuromuscular junction; endosome; receptor complex; intracellular membrane-bound organelle; dendritic spine; extracellular region; axon; apical part of cell; plasma membrane; spindle midzone

Molecular Function: heparin binding; serine-type endopeptidase inhibitor activity; identical protein binding; protein binding; enzyme binding; protease activator activity; DNA binding; transition metal ion binding; PTB domain binding; acetylcholine receptor binding; receptor binding

Biological Process: extracellular matrix organization and biogenesis; adult locomotory behavior; mRNA polyadenylation; locomotory behavior; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; regulation of translation; platelet degranulation; synaptic growth at neuromuscular junction; forebrain development; dendrite development; collateral sprouting in the absence of injury; visual learning; neuromuscular process controlling balance; cell adhesion; neurite development; platelet activation; cholesterol metabolic process; Notch signaling pathway; cellular copper ion homeostasis; regulation of epidermal growth factor receptor activity; axon cargo transport; mating behavior; regulation of multicellular organism growth; endocytosis; axon midline choice point recognition; smooth endoplasmic reticulum calcium ion homeostasis; neuron apoptosis; negative regulation of neuron differentiation; suckling behavior; axonogenesis; ionotropic glutamate receptor signaling pathway; regulation of synapse structure and activity; regulation of protein binding; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; blood coagulation; neuron remodeling

Disease: Alzheimer Disease