Human Apolipoprotein M (APOM) ELISA Kit | APOM elisa kit

Human Apolipoprotein M (APOM) High Sensitive ELISA Kit

Cat. Num. AAA2708099

• Gene Names: APOM; G3a; NG20; apo-M; HSPC336
• Reactivity: Human
• Applications: ELISA
• Synonyms: Apolipoprotein M (APOM); Human Apolipoprotein M (APOM) High Sensitive ELISA Kit; Apolipoprotein M; Apo-M; G3a; NG20; Protein G3a; APOM elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Apolipoprotein M (APOM).; No significant cross-reactivity or interference between High Sensitive Apolipoprotein M (APOM) and analogues was observed.
• Sequence Length: 116

• Applicable Applications for APOM elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.121ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Apolipoprotein M (APOM) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Apolipoprotein M (APOM) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Apolipoprotein M (APOM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Apolipoprotein M (APOM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Apolipoprotein M (APOM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Apolipoprotein M (APOM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 371873523
• NCBI GeneID: 55937
• NCBI Accession #: NP_001243098.1
• NCBI GenBank Nucleotide #: NP_001243098.1
• UniProt Accession #: O95445
• NCBI Official Full Name: apolipoprotein M isoform 2
• NCBI Official Synonym Full Names: apolipoprotein M
• NCBI Official Symbol: APOM
• NCBI Official Synonym Symbols: G3a; NG20; apo-M; HSPC336
• NCBI Protein Information: apolipoprotein M
• UniProt Protein Name: Apolipoprotein M
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOM
• UniProt Synonym Gene Names: G3A; NG20; Apo-M; ApoM
• UniProt Entry Name: APOM_HUMAN

NCBI Description

The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Alternate splicing results in both coding and non-coding variants of this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

APOM: Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid. Plasma protein. Expressed in liver and kidney. Belongs to the calycin superfamily. Lipocalin family. Highly divergent.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: integral to plasma membrane

Molecular Function: lipid transporter activity; antioxidant activity; phospholipid binding

Biological Process: cholesterol homeostasis; reverse cholesterol transport; response to glucose stimulus; cholesterol efflux; lipoprotein metabolic process