Human Apolipoprotein C-I (APOC1) ELISA Kit | APOC1 elisa kit

Human Apolipoprotein C-I (APOC1) ELISA Kit

Cat. Num. AAA162332

• Gene Names: CHM; TCD; GGTA; REP-1; DXS540; HSD-32
• Reactivity: Human
• Synonyms: Apolipoprotein C-I (APOC1); Human Apolipoprotein C-I (APOC1) ELISA Kit; APOC1 elisa kit

• Reactivity: Human
• Sequence Length: 653

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.2?g/ml - 60?g/ml
• Sensitivity: 0.104?g/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOC1 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Apolipoprotein C-I (also known as APOC1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human APOC1 antibody. APOC1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human APOC1 Antibody is added and binds to APOC1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated APOC1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human APOC1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 119618965
• NCBI GeneID: 1121
• UniProt Accession #: P24386; O43732; A1L4D2
• Molecular Weight: 12,301 Da
• NCBI Official Full Name: choroideremia (Rab escort protein 1)
• NCBI Official Synonym Full Names: CHM, Rab escort protein 1
• NCBI Official Symbol: CHM
• NCBI Official Synonym Symbols: TCD; GGTA; REP-1; DXS540; HSD-32
• NCBI Protein Information: rab proteins geranylgeranyltransferase component A 1
• UniProt Protein Name: Rab proteins geranylgeranyltransferase component A 1
• Protein Family: Apolipoprotein
• UniProt Gene Name: CHM
• UniProt Synonym Gene Names: REP1; TCD; REP-1

NCBI Description

This gene encodes component A of the RAB geranylgeranyl transferase holoenzyme. In the dimeric holoenzyme, this subunit binds unprenylated Rab GTPases and then presents them to the catalytic Rab GGTase subunit for the geranylgeranyl transfer reaction. Rab GTPases need to be geranylgeranyled on either one or two cysteine residues in their C-terminus to localize to the correct intracellular membrane. Mutations in this gene are a cause of choroideremia; also known as tapetochoroidal dystrophy (TCD). This X-linked disease is characterized by progressive dystrophy of the choroid, retinal pigment epithelium and retina. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2016]

Uniprot Description

Substrate-binding subunit of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B composed of RABGGTA and RABGGTB, and remains bound to it after the geranylgeranyl transfer reaction. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Besides, a pre-formed complex consisting of CHM and the Rab GGTase dimer (RGGT or component B) can bind to and prenylate Rab proteins; this alternative pathway is proposed to be the predominant pathway for Rab protein geranylgeranylation.