Typical Testing Data/Standard Curve (for reference only)
Human Antioncogene p21 protein ELISA Kit | p21 elisa kit
Human Antioncogene p21 protein ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: P21 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-P21 antibody and an P21-HRP conjugate. The assay sample and buffer are incubated together with P21-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the P21 concentration since P21 from samples and P21-HRP conjugate compete for the anti-P21 antibody binding site. Since the number of sites is limited, as more sites are occupied by P21 from the sample, fewer sites are left to bind P21-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P21 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Nov 2010]
Uniprot Description
p21Cip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. Its expression is tightly controlled by p53, through which this protein mediates the p53-dependent cell cycle arrest at G1 phase.
Protein type: Inhibitor; Cell cycle regulation
Chromosomal Location of Human Ortholog: 6p21.2
Cellular Component: nucleoplasm; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleus; cytosol
Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; cyclin binding; cyclin-dependent protein kinase activating kinase activity; ubiquitin protein ligase binding; metal ion binding; protein complex binding
Biological Process: nerve growth factor receptor signaling pathway; response to arsenic; response to toxin; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; protein amino acid phosphorylation; negative regulation of cell proliferation; positive regulation of fibroblast proliferation; positive regulation of B cell proliferation; G2/M transition of mitotic cell cycle; cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to X-ray; response to corticosterone stimulus; epidermal growth factor receptor signaling pathway; response to drug; organ regeneration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; negative regulation of cyclin-dependent protein kinase activity; response to organic nitrogen; positive regulation of programmed cell death; response to hyperoxia; positive regulation of protein kinase activity; Ras protein signal transduction; cellular response to extracellular stimulus; negative regulation of phosphorylation; innate immune response; regulation of protein import into nucleus, translocation; mitotic cell cycle; negative regulation of cell growth; regulation of cyclin-dependent protein kinase activity; response to DNA damage stimulus; G1/S transition of mitotic cell cycle; negative regulation of apoptosis
Research Articles on p21
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Product Notes
The Human p21 cdkn1a (Catalog #AAA731903) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA731903 ELISA Kit recognizes Human p21. It is sometimes possible for the material contained within the vial of "Antioncogene p21 protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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