Human Anti-Myelin Associated Glycoprotein Antibody (Anti-MAG) ELISA Kit | MAG elisa kit

Human Anti-Myelin Associated Glycoprotein Antibody (Anti-MAG) ELISA Kit

Cat. Num. AAA2097744

• Gene Names: MAG; GMA; S-MAG; SPG75; SIGLEC4A; SIGLEC-4A
• Reactivity: Human
• Synonyms: Anti-Myelin Associated Glycoprotein Antibody (Anti-MAG); Human Anti-Myelin Associated Glycoprotein Antibody (Anti-MAG) ELISA Kit; GMA; S-MAG; Siglec-4; SIGLEC4A; Sialic Acid Binding Ig-Like Lectin 4A; MAG elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Anti-Myelin Associated Glycoprotein Antibody (Anti-MAG).; No significant cross-reactivity or interference between Anti-Myelin Associated Glycoprotein Antibody (Anti-MAG) and analogues was observed.
• Sequence Length: 601

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.59ng/mL
• Application: Enzyme-linked immunosorbent assay for Antibody Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Myelin Associated Glycoprotein Antibody (Anti-MAG) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Myelin Associated Glycoprotein Antibody (Anti-MAG) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAG elisa kit

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Myelin Associated Glycoprotein Antibody (Anti-MAG) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Myelin Associated Glycoprotein Antibody (Anti-MAG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MAG elisa kit

Infection immunity; Neuroscience; Autoimmunity

NCBI and Uniprot Product Information

• NCBI GI #: 312836851
• NCBI GeneID: 4099
• NCBI Accession #: NP_001186145.1
• NCBI GenBank Nucleotide #: NM_001199216.1
• UniProt Accession #: P20916; Q15489; Q567S4; B7Z2E5; F5GYC0
• Molecular Weight: 66,224 Da
• NCBI Official Full Name: myelin-associated glycoprotein isoform c
• NCBI Official Synonym Full Names: myelin associated glycoprotein
• NCBI Official Symbol: MAG
• NCBI Official Synonym Symbols: GMA; S-MAG; SPG75; SIGLEC4A; SIGLEC-4A
• NCBI Protein Information: myelin-associated glycoprotein
• UniProt Protein Name: Myelin-associated glycoprotein
• Protein Family: Allergen
• UniProt Gene Name: MAG
• UniProt Synonym Gene Names: GMA

NCBI Description

The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

MAG: Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3- linked sialic acid. Binds to RTN4R. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.12

Cellular Component: compact myelin; cytoplasm; integral component of plasma membrane; membrane raft; myelin sheath; paranode region of axon; plasma membrane

Molecular Function: carbohydrate binding; ganglioside GT1b binding; protein homodimerization activity; protein kinase binding; receptor binding; sialic acid binding

Biological Process: cell adhesion; cell-cell adhesion via plasma-membrane adhesion molecules; leukocyte migration; myelination in the central nervous system; negative regulation of axon extension; negative regulation of axonogenesis; negative regulation of neuron apoptosis; negative regulation of neuron projection development; positive regulation of astrocyte differentiation; positive regulation of myelination

Disease: Spastic Paraplegia 75, Autosomal Recessive