Human Anti Muellerian hormone type 2 receptor (AMHR2) ELISA Kit | AMHR2 elisa kit

Human Anti Muellerian hormone type 2 receptor (AMHR2) ELISA Kit

Cat. Num. AAA7216385

• Gene Names: AMHR2; AMHR; MRII; MISR2; MISRII
• Reactivity: Human
• Synonyms: Anti Muellerian hormone type 2 receptor (AMHR2); Human Anti Muellerian hormone type 2 receptor (AMHR2) ELISA Kit; AMHR2 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This AMHR2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AMHR2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AMHR2 elisa kit

Principle of the assay: AMHR2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AMHR2 antibody and an AMHR2-HRP conjugate. The assay sample and buffer are incubated together with AMHR2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMHR2 concentration since AMHR2 from samples and AMHR2-HRP conjugate compete for the anti-AMHR2 antibody binding site. Since the number of sites is limited, as more sites are occupied by AMHR2 from the sample, fewer sites are left to bind AMHR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMHR2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for AMHR2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 257743467
• NCBI GeneID: 269
• NCBI Accession #: NP_001158162.1
• NCBI GenBank Nucleotide #: NM_001164690.1
• Molecular Weight: 51,827 Da
• NCBI Official Full Name: anti-Muellerian hormone type-2 receptor isoform 2
• NCBI Official Synonym Full Names: anti-Mullerian hormone receptor, type II
• NCBI Official Symbol: AMHR2
• NCBI Official Synonym Symbols: AMHR; MRII; MISR2; MISRII
• NCBI Protein Information: anti-Muellerian hormone type-2 receptor; AMH type II receptor; MIS type II receptor; anti-Muellerian hormone type II receptor; Mullerian inhibiting substance type II receptor; Muellerian inhibiting substance type II receptor
• UniProt Protein Name: Anti-Muellerian hormone type-2 receptor
• Protein Family: Anti-Muellerian hormone type-2 receptor
• UniProt Gene Name: AMHR2
• UniProt Synonym Gene Names: AMHR; MISR2; AMH type II receptor; MISRII; MRII
• UniProt Entry Name: AMHR2_HUMAN

NCBI Description

This gene encodes the receptor for the anti-Mullerian hormone (AMH) which, in addition to testosterone, results in male sex differentiation. AMH and testosterone are produced in the testes by different cells and have different effects. Testosterone promotes the development of male genitalia while the binding of AMH to the encoded receptor prevents the development of the mullerian ducts into uterus and Fallopian tubes. Mutations in this gene are associated with persistent Mullerian duct syndrome type II. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2009]

Uniprot Description

MISR2: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for anti-Muellerian hormone. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.

Protein type: EC 2.7.11.30; Membrane protein, integral; Protein kinase, TKL; Protein kinase, Ser/Thr (receptor); Kinase, protein; TKL group; STKR family; Type2 subfamily

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: integral to plasma membrane

Molecular Function: protein binding; transforming growth factor beta receptor activity, type II; hormone binding; metal ion binding; receptor activity; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: Mullerian duct regression; transforming growth factor beta receptor signaling pathway; signal transduction; sex differentiation; protein amino acid phosphorylation

Disease: Persistent Mullerian Duct Syndrome, Types I And Ii