Typical Testing Data/Standard Curve (for reference only)
Human Ankyrin repeat domain containing protein 11 (ANKRD11) ELISA Kit | ANKRD11 elisa kit
Human Ankyrin repeat domain containing protein 11 (ANKRD11) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ANKRD11 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ANKRD11 antibody and an ANKRD11-HRP conjugate. The assay sample and buffer are incubated together with ANKRD11-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANKRD11 concentration since ANKRD11 from samples and ANKRD11-HRP conjugate compete for the anti-ANKRD11 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANKRD11 from the sample, fewer sites are left to bind ANKRD11-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANKRD11 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This locus encodes an ankryin repeat domain-containing protein. The encoded protein inhibits ligand-dependent activation of transcription. Mutations in this gene have been associated with KBG syndrome, which is characterized by macrodontia, distinctive craniofacial features, short stature, skeletal anomalies, global developmental delay, seizures and intellectual disability. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 2 and X. [provided by RefSeq, Jan 2012]
Uniprot Description
ANKRD11: May recruit HDACs to the p160 coactivators/nuclear receptor complex to inhibit ligand-dependent transactivation. Defects in ANKRD11 are the cause of KBG syndrome (KBGS). A syndrome characterized by macrodontia of the upper central incisors, distinctive craniofacial findings, short stature, skeletal anomalies, and neurologic involvement that includes global developmental delay, seizures, and intellectual disability.
Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 16q24.3
Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleus
Biological Process: in utero embryonic development; skeletal morphogenesis; multicellular organism growth; tissue homeostasis; odontogenesis of dentine-containing teeth
Disease: Kbg Syndrome
Research Articles on ANKRD11
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Product Notes
The Human ANKRD11 ankrd11 (Catalog #AAA7213868) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7213868 ELISA Kit recognizes Human ANKRD11. It is sometimes possible for the material contained within the vial of "Ankyrin repeat domain containing protein 11 (ANKRD11), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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