Typical Testing Data/Standard Curve (for reference only)
Human Anion exchange protein 2 (SLC4A2) ELISA Kit | SLC4A2 elisa kit
Human Anion exchange protein 2 (SLC4A2) ELISA Kit
Gene Names
SLC4A2; AE2; HKB3; BND3L; NBND3; EPB3L1
Reactivity
Human
Synonyms
Anion exchange protein 2 (SLC4A2); Human Anion exchange protein 2 (SLC4A2) ELISA Kit; SLC4A2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of SLC4A2. No significant cross-reactivity or interference between SLC4A2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SLC4A2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SLC4A2 elisa kit
Intended Uses: This SLC4A2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SLC4A2. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: SLC4A2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SLC4A2 antibody and an SLC4A2-HRP conjugate. The assay sample and buffer are incubated together with SLC4A2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLC4A2 concentration since SLC4A2 from samples and SLC4A2-HRP conjugate compete for the anti-SLC4A2 antibody binding site. Since the number of sites is limited, as more sites are occupied by SLC4A2 from the sample, fewer sites are left to bind SLC4A2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLC4A2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: SLC4A2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SLC4A2 antibody and an SLC4A2-HRP conjugate. The assay sample and buffer are incubated together with SLC4A2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLC4A2 concentration since SLC4A2 from samples and SLC4A2-HRP conjugate compete for the anti-SLC4A2 antibody binding site. Since the number of sites is limited, as more sites are occupied by SLC4A2 from the sample, fewer sites are left to bind SLC4A2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLC4A2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for SLC4A2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
136,247 Da
NCBI Official Full Name
anion exchange protein 2 isoform 1
NCBI Official Synonym Full Names
solute carrier family 4 (anion exchanger), member 2
NCBI Official Symbol
SLC4A2
NCBI Official Synonym Symbols
AE2; HKB3; BND3L; NBND3; EPB3L1
NCBI Protein Information
anion exchange protein 2; AE 2; anion exchanger 2 type a; anion exchanger 2 type b1; anion exchanger 2 type b2; solute carrier family 4 member 2; non-erythroid band 3-like protein; erythrocyte membrane protein band 3-like 1
UniProt Protein Name
Anion exchange protein 2
Protein Family
UniProt Gene Name
SLC4A2
UniProt Synonym Gene Names
AE2; EPB3L1; HKB3; MPB3L; AE 2; Anion exchanger 2; BND3L
UniProt Entry Name
B3A2_HUMAN
Uniprot Description
SLC4A2: Plasma membrane anion exchange protein of wide distribution. Belongs to the anion exchanger (TC 2.A.31) family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter; Membrane protein, integral; Membrane protein, multi-pass; Transporter, SLC family
Chromosomal Location of Human Ortholog: 7q36.1
Cellular Component: focal adhesion; membrane; integral to plasma membrane; basolateral plasma membrane; plasma membrane
Molecular Function: chloride transmembrane transporter activity; inorganic anion exchanger activity; anion transmembrane transporter activity
Biological Process: bicarbonate transport; regulation of intracellular pH; ion transport; transmembrane transport; anion transport
Research Articles on SLC4A2
Similar Products
Product Notes
The Human SLC4A2 slc4a2 (Catalog #AAA7222302) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7222302 ELISA Kit recognizes Human SLC4A2. It is sometimes possible for the material contained within the vial of "Anion exchange protein 2 (SLC4A2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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