Typical Testing Data/Standard Curve (for reference only)
Human AMP deaminase 3 (AMPD3) ELISA Kit | AMPD3 elisa kit
Human AMP deaminase 3 (AMPD3) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AMPD3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AMPD3 antibody and an AMPD3-HRP conjugate. The assay sample and buffer are incubated together with AMPD3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMPD3 concentration since AMPD3 from samples and AMPD3-HRP conjugate compete for the anti-AMPD3 antibody binding site. Since the number of sites is limited, as more sites are occupied by AMPD3 from the sample, fewer sites are left to bind AMPD3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMPD3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the AMP deaminase gene family. The encoded protein is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. This gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. Mutations in this gene lead to the clinically asymptomatic, autosomal recessive condition erythrocyte AMP deaminase deficiency. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
AMPD3: AMP deaminase plays a critical role in energy metabolism. Defects in AMPD3 are the cause of adenosine monophosphate deaminase deficiency erythrocyte type (AMPDDE); also known as erythrocyte AMP deaminase deficiency. AMPDDE is a metabolic disorder due to lack of activity of the erythrocyte isoform of AMP deaminase. It is a clinically asymptomatic condition characterized by a 50% increase in steady-state levels of ATP in affected cells. Individuals with complete deficiency of erythrocyte AMP deaminase are healthy and have no hematologic disorders. Belongs to the adenosine and AMP deaminases family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.5.4.6; Hydrolase; Nucleotide Metabolism - purine
Chromosomal Location of Human Ortholog: 11p15
Cellular Component: cytosol
Molecular Function: metal ion binding; AMP deaminase activity
Biological Process: ATP metabolic process; IMP salvage; nucleobase, nucleoside and nucleotide metabolic process; ADP metabolic process; purine base metabolic process; purine salvage; AMP catabolic process
Disease: Erythrocyte Amp Deaminase Deficiency
Research Articles on AMPD3
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Product Notes
The Human AMPD3 ampd3 (Catalog #AAA7217226) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7217226 ELISA Kit recognizes Human AMPD3. It is sometimes possible for the material contained within the vial of "AMP deaminase 3 (AMPD3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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