Typical Testing Data/Standard Curve (for reference only)
Human Amelotin (AMTN) ELISA Kit | AMTN elisa kit
Human Amelotin (AMTN) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AMTN ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AMTN antibody and an AMTN-HRP conjugate. The assay sample and buffer are incubated together with AMTN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMTN concentration since AMTN from samples and AMTN-HRP conjugate compete for the anti-AMTN antibody binding site. Since the number of sites is limited, as more sites are occupied by AMTN from the sample, fewer sites are left to bind AMTN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMTN concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The mineralized portions of teeth, the dentin and enamel, are formed by mesenchyme-derived odontoblasts and epithelium-derived ameloblasts, respectively. As ameloblasts differentiate, they deposit specific proteins necessary for enamel formation, including amelogenin (AMELX; MIM 300391), enamelin (ENAM; MIM 606585), and ameloblastin (AMBN; MIM 601259), in the organic enamel matrix. Amelotin is specifically expressed in maturation-stage ameloblasts (Iwasaki et al., 2005 [PubMed 16304441]).[supplied by OMIM, Mar 2008]
Uniprot Description
AMTN: Could be a cell adhesion protein involved in the maturation of tooth enamel. Belongs to the amelotin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide; Cell adhesion; Extracellular matrix
Chromosomal Location of Human Ortholog: 4q13.3
Cellular Component: proteinaceous extracellular matrix; basal lamina; intercellular junction
Biological Process: biomineral formation; cell adhesion; odontogenesis of dentine-containing teeth
Similar Products
Product Notes
The Human AMTN amtn (Catalog #AAA7233819) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7233819 ELISA Kit recognizes Human AMTN. It is sometimes possible for the material contained within the vial of "Amelotin (AMTN), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
