Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Alpha-N-acetylglucosaminidase (alpha-NAG) Standard Curve.)
Human Alpha-N-Acetylglucosaminidase (alpha-NAG) ELISA Kit | alpha-NAG elisa kit
Human Alpha-N-Acetylglucosaminidase (alpha-NAG) ELISA Kit
Gene Names
NAGLU; NAG; CMT2V; MPS3B; UFHSD; MPS-IIIB
Reactivity
Human
Synonyms
Alpha-N-Acetylglucosaminidase (alpha-NAG); Human Alpha-N-Acetylglucosaminidase (alpha-NAG) ELISA Kit; NAGLU; MPS-IIIB; MPS3B; NAG; UFHSD; N-acetyl-alpha-glucosaminidase; alpha-N-acetylglucosaminidase; alpha-NAG elisa kit
Reactivity
Human
Specificity
Human Alpha-N-acetylglucosaminidase (alpha-NAG) ELISA Kit has high sensitivity and excellent specificity for detection of Human NAGLU. No significant cross-reactivity or interference between Human NAGLU and analogues was observed.
Sequence Length
743
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Alpha-N-acetylglucosaminidase (alpha-NAG) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Alpha-N-acetylglucosaminidase (alpha-NAG) Standard Curve.)
Related Product Information for alpha-NAG elisa kit
Description: This Human Alpha-N-acetylglucosaminidase (alpha-NAG) ELISA Kit employs a two-site sandwich ELISA to quantitate NAGLU in samples. An antibody specific for NAGLU has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAGLU present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAGLU is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAGLU bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Alpha-N-acetylgalactosaminidase (EC 3.2.1.49) is a lysosomal glycohydrolase that cleaves alpha-N-acetylgalactosaminyl moieties from glycoconjugates. The cDNA encodes a 411-amino acid protein with a 17-residue signal peptide and 6 putative N-glycosylation sites. Northern blot analysis detected 2 mRNA transcripts of 3.6 and 2.2 kb. Sequence analysis revealed striking similarities between the NAGA gene and exons 1-6 of the alpha-galactosidase A gene (GLA), suggesting that the 2 genes evolved by duplication and divergence from a common ancestral locus. Wang and Desnick (1991) also pointed to remarkable amino acid identity between the NAGA and GLA genes.
Background: Alpha-N-acetylgalactosaminidase (EC 3.2.1.49) is a lysosomal glycohydrolase that cleaves alpha-N-acetylgalactosaminyl moieties from glycoconjugates. The cDNA encodes a 411-amino acid protein with a 17-residue signal peptide and 6 putative N-glycosylation sites. Northern blot analysis detected 2 mRNA transcripts of 3.6 and 2.2 kb. Sequence analysis revealed striking similarities between the NAGA gene and exons 1-6 of the alpha-galactosidase A gene (GLA), suggesting that the 2 genes evolved by duplication and divergence from a common ancestral locus. Wang and Desnick (1991) also pointed to remarkable amino acid identity between the NAGA and GLA genes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
alpha-N-acetylglucosaminidase
NCBI Official Synonym Full Names
N-acetyl-alpha-glucosaminidase
NCBI Official Symbol
NAGLU
NCBI Official Synonym Symbols
NAG; CMT2V; MPS3B; UFHSD; MPS-IIIB
NCBI Protein Information
alpha-N-acetylglucosaminidase
UniProt Protein Name
Alpha-N-acetylglucosaminidase
UniProt Gene Name
NAGLU
UniProt Synonym Gene Names
UFHSD1; NAG
UniProt Entry Name
ANAG_HUMAN
NCBI Description
This gene encodes an enzyme that degrades heparan sulfate by hydrolysis of terminal N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides. Defects in this gene are the cause of mucopolysaccharidosis type IIIB (MPS-IIIB), also known as Sanfilippo syndrome B. This disease is characterized by the lysosomal accumulation and urinary excretion of heparan sulfate. [provided by RefSeq, Jul 2008]
Research Articles on alpha-NAG
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Product Notes
The Human alpha-NAG naglu (Catalog #AAA9715885) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715885 ELISA Kit recognizes Human alpha-NAG. It is sometimes possible for the material contained within the vial of "Alpha-N-Acetylglucosaminidase (alpha-NAG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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