Human alpha 1-Microglobulin ELISA Kit | AMBP elisa kit
Human alpha 1-Microglobulin ELISA Kit
Gene Names
AMBP; A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC; AMBP
Reactivity
Human
Synonyms
alpha 1-Microglobulin; Human alpha 1-Microglobulin ELISA Kit; Human alpha 1-Microglobulin; AMBP elisa kit
Reactivity
Human
Samples
Human biological samples
Assay Type
Sandwich
Preparation and Storage
Store at 4 degree C.
Related Product Information for AMBP elisa kit
Intended Uses: The A1M test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring A1M in human biological samples. If the ELISA is to be used outside the intended use, the user may need to optimize for said use.
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Alpha 1-Microglobulin (A1M) present in samples reacts with the anti-A1M antibodies, which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-A1M antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound A1M. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of A1M in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of A1M in the test sample. The quantity of A1M in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Alpha 1-Microglobulin (A1M) present in samples reacts with the anti-A1M antibodies, which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-A1M antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound A1M. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of A1M in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of A1M in the test sample. The quantity of A1M in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Product Categories/Family for AMBP elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
38,999 Da
NCBI Official Full Name
alpha-1-microglobulin
NCBI Official Synonym Full Names
alpha-1-microglobulin/bikunin precursor
NCBI Official Symbol
AMBP
NCBI Official Synonym Symbols
A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC; AMBP
NCBI Protein Information
protein AMBP; bikunin; uristatin; protein HC; trypstatin; uronic-acid-rich protein; growth-inhibiting protein 19; inter-alpha-trypsin inhibitor light chain; complex-forming glycoprotein heterogeneous in charge
UniProt Protein Name
Protein AMBP
Protein Family
UniProt Gene Name
AMBP
UniProt Synonym Gene Names
HCP; ITIL
UniProt Entry Name
AMBP_HUMAN
NCBI Description
This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq]
Uniprot Description
AMBP: Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 9q32-q33
Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; extracellular region; plasma membrane
Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; IgA binding; calcium oxalate binding; heme binding; calcium channel inhibitor activity
Biological Process: receptor-mediated endocytosis; negative regulation of JNK cascade; viral reproduction; heme catabolic process; negative regulation of immune response; protein catabolic process; female pregnancy; cell adhesion; protein-chromophore linkage
Research Articles on AMBP
1. increased protein levels in preeclamptic women
2. significant relationship was established between the low level and absence of bikunin, and pro-u-PA in urine samples from patients with bladder tumors; identified bikunin loss in urine as a potential bladder carcinoma marker
3. Overexpression of Urinary trypsin inhibitor is associated with recurrence in hepatocellular carcinoma.
4. The transfer of heavy chains from bikunin proteins to hyaluronan requires both TSG-6 and HC2.
5. Overexpression of bikunin reduced the gene expression of matriptase, which attenuated in vitro cell invasion in prostate cancer.
6. In the present study, structural motifs regulating bikunin's anti-inflammatory function are delineated. Binding of bikunin to neutrophils is critically important for its anti-inflammatory capacity.
7. urinary trypsin inhibitor is insufficiently produced for neutrophil elastase release after cardiac arrest
8. in multiple myeloma patients without renal failure, elevated Alpha-1-microglobulin excretion might be an early marker of renal tubular involvement
9. AMBP has radical scavenging activity.
10. Bikunin was found to localize on the cell membrane, while tryptase was in the secretary granules of the mast cells from psoriatic lesions.
11. Free uristatin and bikunin pass readily into urine and are primarily bound to heavy chains that constitute the proinhibitor form in plasma.
12. small angle X-ray scanning study of the monomer and dimer forms of alpha1-microglobulin; monomer is characterised by a radius of gyration R(G)= 2.20 nm and D(max)=6.3 nm and the dimer by R(G)=2.99 nm and D(max)=9.5 nm
13. the second processed protein from AMBP, bikunin, strongly interacted with the full-length hepatitis E virus ORF3 protein
14. Processing of the lipocalin alpha(1)-microglobulin by hemoglobin induces heme-binding and heme-degradation properties.
15. Identification of the protein bikunin as an endogenous, competitive inhibitor of a dynorphin-converting enzyme in human cerebrospinal fluid.
16. alpha-1-microglobulin is covalently bound to kynurenine-derived chromophores
17. Data show that alpha(1) -microglobulin/bikunin precursor specifically interacts with the ORF3 protein of hepatitis E virus and has a direct role in enhancing alpha(1)microglobulin export from the hepatocyte.
18. the ORF3 protein exploits the endosomal sorting machinery to enhance the secretion of an immunosuppressant molecule (alpha1 microglobulin) from cultured hepatocytes
19. The ability of alpha-1-microglobulin to restore the initial conformation characteristic for the native protein and the internal dynamics after the unfolding of the globule by 10 M urea and 6 M guanidine hydrochloride has been ascertained.
20. Transfection into H460M Human large cell lung carcinoma line decreased tumor growth and metastasis in nude mice
2. significant relationship was established between the low level and absence of bikunin, and pro-u-PA in urine samples from patients with bladder tumors; identified bikunin loss in urine as a potential bladder carcinoma marker
3. Overexpression of Urinary trypsin inhibitor is associated with recurrence in hepatocellular carcinoma.
4. The transfer of heavy chains from bikunin proteins to hyaluronan requires both TSG-6 and HC2.
5. Overexpression of bikunin reduced the gene expression of matriptase, which attenuated in vitro cell invasion in prostate cancer.
6. In the present study, structural motifs regulating bikunin's anti-inflammatory function are delineated. Binding of bikunin to neutrophils is critically important for its anti-inflammatory capacity.
7. urinary trypsin inhibitor is insufficiently produced for neutrophil elastase release after cardiac arrest
8. in multiple myeloma patients without renal failure, elevated Alpha-1-microglobulin excretion might be an early marker of renal tubular involvement
9. AMBP has radical scavenging activity.
10. Bikunin was found to localize on the cell membrane, while tryptase was in the secretary granules of the mast cells from psoriatic lesions.
11. Free uristatin and bikunin pass readily into urine and are primarily bound to heavy chains that constitute the proinhibitor form in plasma.
12. small angle X-ray scanning study of the monomer and dimer forms of alpha1-microglobulin; monomer is characterised by a radius of gyration R(G)= 2.20 nm and D(max)=6.3 nm and the dimer by R(G)=2.99 nm and D(max)=9.5 nm
13. the second processed protein from AMBP, bikunin, strongly interacted with the full-length hepatitis E virus ORF3 protein
14. Processing of the lipocalin alpha(1)-microglobulin by hemoglobin induces heme-binding and heme-degradation properties.
15. Identification of the protein bikunin as an endogenous, competitive inhibitor of a dynorphin-converting enzyme in human cerebrospinal fluid.
16. alpha-1-microglobulin is covalently bound to kynurenine-derived chromophores
17. Data show that alpha(1) -microglobulin/bikunin precursor specifically interacts with the ORF3 protein of hepatitis E virus and has a direct role in enhancing alpha(1)microglobulin export from the hepatocyte.
18. the ORF3 protein exploits the endosomal sorting machinery to enhance the secretion of an immunosuppressant molecule (alpha1 microglobulin) from cultured hepatocytes
19. The ability of alpha-1-microglobulin to restore the initial conformation characteristic for the native protein and the internal dynamics after the unfolding of the globule by 10 M urea and 6 M guanidine hydrochloride has been ascertained.
20. Transfection into H460M Human large cell lung carcinoma line decreased tumor growth and metastasis in nude mice
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Product Notes
The Human AMBP ambp (Catalog #AAA564034) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA564034 ELISA Kit recognizes Human AMBP. It is sometimes possible for the material contained within the vial of "alpha 1-Microglobulin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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