Human Alpha 1-Acid Glycoprotein ELISA Kit | Orm1 elisa kit
Human alpha 1-Acid Glycoprotein ELISA Kit
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the alpha 1-Acid Glycoprotein (AGP) present in samples reacts with the anti-AGP antibodies, which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-AGP antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound AGP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of AGP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of AGP in the test sample. The quantity of AGP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
NCBI and Uniprot Product Information
NCBI Description
an acute phase reactant protein; may have a role in the acute inflammation response [RGD]
Uniprot Description
ORM1: Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction. Belongs to the calycin superfamily. Lipocalin family.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space
Molecular Function: drug binding
Biological Process: response to drug; organ regeneration; response to vitamin E; regulation of immune system process; transport; acute-phase response; response to lipopolysaccharide; chronic inflammatory response to antigenic stimulus; response to organic cyclic substance; acute inflammatory response to antigenic stimulus
Research Articles on Orm1
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Product Notes
The Human Orm1 orm1 (Catalog #AAA564030) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA564030 ELISA Kit recognizes Human Orm1. It is sometimes possible for the material contained within the vial of "Alpha 1-Acid Glycoprotein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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