Typical Testing Data/Standard Curve (for reference only)
Human Allergin 1 (C17orf60) ELISA Kit | C17orf60 elisa kit
Human Allergin 1 (C17orf60) ELISA Kit
Gene Names
MILR1; MCA32; MCA-32; C17orf60; Allergin-1
Reactivity
Human
Synonyms
Allergin 1 (C17orf60); Human Allergin 1 (C17orf60) ELISA Kit; C17orf60 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of C17orf60. No significant cross-reactivity or interference between C17orf60 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C17orf60 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for C17orf60 elisa kit
Intended Uses: This C17orf60 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human C17orf60. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: C17orf60 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-C17orf60 antibody and an C17orf60-HRP conjugate. The assay sample and buffer are incubated together with C17orf60-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C17orf60 concentration since C17orf60 from samples and C17orf60-HRP conjugate compete for the anti-C17orf60 antibody binding site. Since the number of sites is limited, as more sites are occupied by C17orf60 from the sample, fewer sites are left to bind C17orf60-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C17orf60 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: C17orf60 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-C17orf60 antibody and an C17orf60-HRP conjugate. The assay sample and buffer are incubated together with C17orf60-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C17orf60 concentration since C17orf60 from samples and C17orf60-HRP conjugate compete for the anti-C17orf60 antibody binding site. Since the number of sites is limited, as more sites are occupied by C17orf60 from the sample, fewer sites are left to bind C17orf60-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C17orf60 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,656 Da
NCBI Official Full Name
allergin-1 isoform Allergin-1L
NCBI Official Synonym Full Names
mast cell immunoglobulin-like receptor 1
NCBI Official Symbol
MILR1
NCBI Official Synonym Symbols
MCA32; MCA-32; C17orf60; Allergin-1
NCBI Protein Information
allergin-1; mast cell antigen 32; allergy inhibitory receptor 1; probable mast cell antigen 32 homolog
UniProt Protein Name
Allergin-1
UniProt Gene Name
MILR1
UniProt Synonym Gene Names
C17orf60; MCA32; MCA-32
UniProt Entry Name
MILR1_HUMAN
Uniprot Description
MCA32: Immunoglobulin-like receptor which plays an inhibitory role in degranulation of mast cells. Negatively regulates IgE- mediated mast cell activation and suppresses the type I immediate hypersensitivity reaction. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 17q23.3
Cellular Component: mast cell granule; integral to plasma membrane
Biological Process: negative regulation of mast cell activation; mast cell degranulation
Research Articles on C17orf60
Similar Products
Product Notes
The Human C17orf60 milr1 (Catalog #AAA7211956) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7211956 ELISA Kit recognizes Human C17orf60. It is sometimes possible for the material contained within the vial of "Allergin 1 (C17orf60), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
