Typical Testing Data/Standard Curve (for reference only)
Human Alkaline ceramidase 3 (ACER3) ELISA Kit | ACER3 elisa kit
Human Alkaline ceramidase 3 (ACER3) ELISA Kit
Gene Names
ACER3; APHC; PHCA
Reactivity
Human
Synonyms
Alkaline ceramidase 3 (ACER3); Human Alkaline ceramidase 3 (ACER3) ELISA Kit; ACER3 elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
100-2500pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ACER3 elisa kit
Intended Uses: This ACER3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ACER3. This ELISA kit for research use only!
Principle of the Assay: ACER3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACER3 antibody and an ACER3-HRP conjugate. The assay sample and buffer are incubated together with ACER3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACER3 concentration since ACER3 from samples and ACER3-HRP conjugate compete for the anti-ACER3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACER3 from the sample, fewer sites are left to bind ACER3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACER3 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ACER3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACER3 antibody and an ACER3-HRP conjugate. The assay sample and buffer are incubated together with ACER3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACER3 concentration since ACER3 from samples and ACER3-HRP conjugate compete for the anti-ACER3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACER3 from the sample, fewer sites are left to bind ACER3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACER3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
15,895 Da
NCBI Official Full Name
Alkaline ceramidase 3
NCBI Official Synonym Full Names
alkaline ceramidase 3
NCBI Official Symbol
ACER3
NCBI Official Synonym Symbols
APHC; PHCA
NCBI Protein Information
alkaline ceramidase 3; alkCDase 3; alkaline CDase 3; alkaline phytoceramidase; phytoceramidase, alkaline; alkaline dihydroceramidase SB89
UniProt Protein Name
Alkaline ceramidase 3
Protein Family
UniProt Gene Name
ACER3
UniProt Synonym Gene Names
APHC; PHCA; AlkCDase 3; Alkaline CDase 3; aPHC
UniProt Entry Name
ACER3_HUMAN
Uniprot Description
ACER3: Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity. Belongs to the alkaline ceramidase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase; Membrane protein, multi-pass; Membrane protein, integral; EC 3.5.1.-; Lipid Metabolism - sphingolipid
Chromosomal Location of Human Ortholog: 11q13.5
Cellular Component: endoplasmic reticulum membrane; integral to Golgi membrane; integral to endoplasmic reticulum membrane
Biological Process: sphingolipid metabolic process; sphingolipid biosynthetic process; positive regulation of cell proliferation; sphingosine biosynthetic process; ceramide metabolic process
Research Articles on ACER3
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Product Notes
The Human ACER3 acer3 (Catalog #AAA7225385) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7225385 ELISA Kit recognizes Human ACER3. It is sometimes possible for the material contained within the vial of "Alkaline ceramidase 3 (ACER3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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