Typical Testing Data/Standard Curve (for reference only)
Human Alkaline ceramidase 1 (ACER1) ELISA Kit | ACER1 elisa kit
Human Alkaline ceramidase 1 (ACER1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ACER1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACER1 antibody and an ACER1-HRP conjugate. The assay sample and buffer are incubated together with ACER1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACER1 concentration since ACER1 from samples and ACER1-HRP conjugate compete for the anti-ACER1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACER1 from the sample, fewer sites are left to bind ACER1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACER1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Ceramides are synthesized during epidermal differentiation and accumulate within the interstices of the stratum corneum, where they represent critical components of the epidermal permeability barrier. Excess cellular ceramide can trigger antimitogenic signals and induce apoptosis, and the ceramide metabolites sphingosine and sphingosine-1-phosphate (S1P) are important bioregulatory molecules. Ceramide hydrolysis in the nucleated cell layers regulates keratinocyte proliferation and apoptosis in response to external stress. Ceramide hydrolysis also occurs at the stratum corneum, releasing free sphingoid base that functions as an endogenous antimicrobial agent. ACER1 is highly expressed in epidermis and catalyzes the hydrolysis of very long chain ceramides to generate sphingosine (Houben et al., 2006 [PubMed 16477081]; Sun et al., 2008 [PubMed 17713573]).[supplied by OMIM, Jul 2010]
Uniprot Description
ACER1: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 8.0. Has a highly restricted substrate specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine but not D-ribo- phytosphingosine or D-erythro-dihydrosphingosine as a backbone. May have a role in regulating the levels of bioactive lipids ceramide and sphingosine 1-phosphate, as well as complex sphingolipids. Belongs to the alkaline ceramidase family.
Protein type: Membrane protein, integral; Hydrolase; Membrane protein, multi-pass; EC 3.5.1.23; Lipid Metabolism - sphingolipid; Endoplasmic reticulum
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane
Molecular Function: ceramidase activity
Biological Process: keratinocyte differentiation; epidermis development; regulation of lipid metabolic process; sphingolipid metabolic process; response to alkalinity; sphingolipid biosynthetic process; sphingosine biosynthetic process; ceramide catabolic process; cell differentiation
Research Articles on ACER1
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Product Notes
The Human ACER1 acer1 (Catalog #AAA7202821) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7202821 ELISA Kit recognizes Human ACER1. It is sometimes possible for the material contained within the vial of "Alkaline ceramidase 1 (ACER1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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