Typical Testing Data/Standard Curve (for reference only)
Human Aldehyde dehydrogenase X, mitochondrial (ALDH1B1) ELISA Kit | ALDH1B1 elisa kit
Human Aldehyde dehydrogenase X, mitochondrial (ALDH1B1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ALDH1B1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ALDH1B1 antibody and an ALDH1B1-HRP conjugate. The assay sample and buffer are incubated together with ALDH1B1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALDH1B1 concentration since ALDH1B1 from samples and ALDH1B1-HRP conjugate compete for the anti-ALDH1B1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ALDH1B1 from the sample, fewer sites are left to bind ALDH1B1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALDH1B1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. This gene does not contain introns in the coding sequence. The variation of this locus may affect the development of alcohol-related problems. [provided by RefSeq, Jul 2008]
Uniprot Description
ALDH1B1: ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. Belongs to the aldehyde dehydrogenase family.
Protein type: Secondary Metabolites Metabolism - limonene and pinene degradation; Other Amino Acids Metabolism - beta-alanine; Amino Acid Metabolism - arginine and proline; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - butanoate; EC 1.2.1.3; Amino Acid Metabolism - histidine; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate; Lipid Metabolism - fatty acid; Oxidoreductase; Carbohydrate Metabolism - ascorbate and aldarate; Amino Acid Metabolism - lysine degradation; Mitochondrial; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Amino Acid Metabolism - tryptophan; Lipid Metabolism - glycerolipid
Chromosomal Location of Human Ortholog: 9p11.1
Cellular Component: nucleoplasm; mitochondrion; intracellular membrane-bound organelle; mitochondrial matrix
Molecular Function: aldehyde dehydrogenase (NAD) activity
Biological Process: ethanol catabolic process; carbohydrate metabolic process
Research Articles on ALDH1B1
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Product Notes
The Human ALDH1B1 aldh1b1 (Catalog #AAA7227449) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7227449 ELISA Kit recognizes Human ALDH1B1. It is sometimes possible for the material contained within the vial of "Aldehyde dehydrogenase X, mitochondrial (ALDH1B1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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