Human Adenylyl Cyclase Associated Protein 2 (CAP2) ELISA Kit | CAP2 elisa kit

Human Adenylyl Cyclase Associated Protein 2 (CAP2) ELISA Kit

Cat. Num. AAA2021482

• Reactivity: Human
• Synonyms: Adenylyl Cyclase Associated Protein 2 (CAP2); Human Adenylyl Cyclase Associated Protein 2 (CAP2) ELISA Kit; CAP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adenylyl Cyclase Associated Protein 2 (CAP2).; No significant cross-reactivity or interference between Adenylyl Cyclase Associated Protein 2 (CAP2) and analogues was observed.
• Sequence Length: 217

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.065ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylyl Cyclase Associated Protein 2 (CAP2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylyl Cyclase Associated Protein 2 (CAP2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAP2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylyl Cyclase Associated Protein 2 (CAP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylyl Cyclase Associated Protein 2 (CAP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylyl Cyclase Associated Protein 2 (CAP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenylyl Cyclase Associated Protein 2 (CAP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CAP2 elisa kit

Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 5453593
• NCBI GeneID: 10486
• NCBI Accession #: NP_006357.1
• NCBI GenBank Nucleotide #: NM_006366.2
• UniProt Accession #: P40123; Q6IAY2; B2R5Y3; B7Z1C4; B7Z214
• Molecular Weight: 24,018 Da
• NCBI Official Full Name: adenylyl cyclase-associated protein 2
• NCBI Official Synonym Full Names: CAP, adenylate cyclase-associated protein, 2 (yeast)
• NCBI Official Symbol: CAP2
• NCBI Protein Information: adenylyl cyclase-associated protein 2
• UniProt Protein Name: Adenylyl cyclase-associated protein 2
• Protein Family: Adenylyl cyclase-associated protein
• UniProt Gene Name: CAP2
• UniProt Synonym Gene Names: CAP 2
• UniProt Entry Name: CAP2_HUMAN

NCBI Description

This gene was identified by its similarity to the gene for human adenylyl cyclase-associated protein. The function of the protein encoded by this gene is unknown. However, the protein appears to be able to interact with adenylyl cyclase-associated protein and actin. [provided by RefSeq, Jul 2008]

Uniprot Description

CAP2: a cyclase-associated protein. CAPs are evolutionarily conserved proteins with roles in regulating the actin cytoskeleton and in signal transduction. CAP2 is expressed mainly in brain, heart and skeletal muscle, and skin. It is found in the nucleus in undifferentiated myoblasts and at the M-line of differentiated myotubes. Up-regulated in human cancers. May play a role in multistage hepatocarcinogenesis.

Protein type: Actin-binding

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: plasma membrane

Molecular Function: actin binding

Biological Process: axon guidance; adenylate cyclase activation; establishment and/or maintenance of cell polarity; cytoskeleton organization and biogenesis; signal transduction