Human Adenylate kinase 7 (AK7) ELISA Kit | AK7 elisa kit

Human Adenylate kinase 7 (AK7) ELISA Kit

Cat. Num. AAA7239878

• Gene Names: AK7; AK 7
• Reactivity: Human
• Synonyms: Adenylate kinase 7 (AK7); Human Adenylate kinase 7 (AK7) ELISA Kit; AK7 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AK7 elisa kit

Intended Uses: This AK7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AK7. This ELISA kit for research use only!

Principle of the Assay: AK7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AK7 antibody and an AK7-HRP conjugate. The assay sample and buffer are incubated together with AK7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AK7 concentration since AK7 from samples and AK7-HRP conjugate compete for the anti-AK7 antibody binding site. Since the number of sites is limited, as more sites are occupied by AK7 from the sample, fewer sites are left to bind AK7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AK7 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 148727333
• NCBI GeneID: 122481
• NCBI Accession #: NP_689540.2
• NCBI GenBank Nucleotide #: NM_152327.3
• Molecular Weight: 82,658 Da
• NCBI Official Full Name: adenylate kinase 7
• NCBI Official Synonym Full Names: adenylate kinase 7
• NCBI Official Symbol: AK7
• NCBI Official Synonym Symbols: AK 7
• NCBI Protein Information: adenylate kinase 7; putative adenylate kinase 7; ATP-AMP transphosphorylase 7
• UniProt Protein Name: Adenylate kinase 7
• Protein Family: Adenylate kinase
• UniProt Gene Name: AK7
• UniProt Synonym Gene Names: AK 7
• UniProt Entry Name: KAD7_HUMAN

Uniprot Description

AK7: Adenylate kinase involved in maintaining ciliary structure and function. Has highest activity toward AMP, and weaker activity toward dAMP, CMP and dCMP.

Protein type: EC 2.7.4.3; Nucleotide Metabolism - purine; EC 2.7.4.6; Kinase, other

Chromosomal Location of Human Ortholog: 14q32.2

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: nucleoside diphosphate kinase activity; adenylate kinase activity; cytidylate kinase activity; ATP binding

Biological Process: inflammatory response to antigenic stimulus; nucleoside triphosphate biosynthetic process; nucleoside diphosphate phosphorylation; spermatogenesis; brain development; axoneme biogenesis