Human Adaptor Related Protein Complex 2 Mu 1 (AP2m1) ELISA Kit | AP2m1 elisa kit

Human Adaptor Related Protein Complex 2 Mu 1 (AP2m1) ELISA Kit

Cat. Num. AAA2022800

• Gene Names: AP2M1; mu2; AP50; CLAPM1
• Reactivity: Human
• Synonyms: Adaptor Related Protein Complex 2 Mu 1 (AP2m1); Human Adaptor Related Protein Complex 2 Mu 1 (AP2m1) ELISA Kit; AP50; CLAPM1; Clathrin-Associated/Assembly/Adaptor Protein; Medium 1; Plasma Membrane Adaptor AP-2 50kDA Protein; Clathrin Coat Adaptor Protein AP50; AP2m1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adaptor Related Protein Complex 2 Mu 1 (AP2m1).; No significant cross-reactivity or interference between Adaptor Related Protein Complex 2 Mu 1 (AP2m1) and analogues was observed.
• Sequence Length: 433

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adaptor Related Protein Complex 2 Mu 1 (AP2m1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adaptor Related Protein Complex 2 Mu 1 (AP2m1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AP2m1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adaptor Related Protein Complex 2 Mu 1 (AP2m1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adaptor Related Protein Complex 2 Mu 1 (AP2m1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adaptor Related Protein Complex 2 Mu 1 (AP2m1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adaptor Related Protein Complex 2 Mu 1 (AP2m1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for AP2m1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 15559334
• NCBI GeneID: 1173
• Molecular Weight: 49,389 Da
• NCBI Official Full Name: Adaptor-related protein complex 2, mu 1 subunit
• NCBI Official Synonym Full Names: adaptor-related protein complex 2, mu 1 subunit
• NCBI Official Symbol: AP2M1
• NCBI Official Synonym Symbols: mu2; AP50; CLAPM1
• NCBI Protein Information: AP-2 complex subunit mu
• UniProt Protein Name: AP-2 complex subunit mu
• UniProt Gene Name: AP2M1
• UniProt Synonym Gene Names: CLAPM1; KIAA0109
• UniProt Entry Name: AP2M1_HUMAN

NCBI Description

This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]

Uniprot Description

AP2M1: a protein of the adaptor complexes medium subunit family. A subunit of the heterotetrameric coat assembly protein complex 2 (AP2) which links clathrin to receptors in coated vesicles. Interacts with the cytoplasmic tails of membrane proteins and polyphosphoinositide-containing lipids. Is not required for clathrin-coated vesicle formation at the plasma membrane, but that it is one of several endocytic adaptors required for the uptake of certain cargo proteins. Is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP-2 is a heterotetramer composed of two large chains (alpha and beta), a medium chain (AP50) and a small chain (AP17).

Protein type: Adaptor/scaffold; Vesicle

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: mitochondrion; lysosomal membrane; plasma membrane; AP-2 adaptor complex; cytosol

Molecular Function: protein binding; low-density lipoprotein receptor binding; signal sequence binding; transporter activity; lipid binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; synaptic transmission; axon guidance; intracellular protein transport; nerve growth factor receptor signaling pathway; viral reproduction; ephrin receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; regulation of defense response to virus by virus