Human Adapter molecule crk (CRK) ELISA Kit | CRK elisa kit

Human Adapter molecule crk (CRK) ELISA Kit

Cat. Num. AAA7203728

• Gene Names: CRK; p38; CRKII
• Reactivity: Human
• Synonyms: Adapter molecule crk (CRK); Human Adapter molecule crk (CRK) ELISA Kit; CRK elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This CRK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CRK. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRK elisa kit

Principle of the assay: CRK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CRK antibody and an CRK-HRP conjugate. The assay sample and buffer are incubated together with CRK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CRK concentration since CRK from samples and CRK-HRP conjugate compete for the anti-CRK antibody binding site. Since the number of sites is limited, as more sites are occupied by CRK from the sample, fewer sites are left to bind CRK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRK concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CRK elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 41327712
• NCBI GeneID: 1398
• NCBI Accession #: NP_058431.2
• NCBI GenBank Nucleotide #: NM_016823.3
• Molecular Weight: 22,906 Da
• NCBI Official Full Name: adapter molecule crk isoform a
• NCBI Official Synonym Full Names: v-crk avian sarcoma virus CT10 oncogene homolog
• NCBI Official Symbol: CRK
• NCBI Official Synonym Symbols: p38; CRKII
• NCBI Protein Information: adapter molecule crk; proto-oncogene c-Crk
• UniProt Protein Name: Adapter molecule crk
• Protein Family: Adapter molecule
• UniProt Gene Name: CRK
• UniProt Entry Name: CRK_HUMAN

NCBI Description

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

CRK: an adaptor protein with an SH2-SH3-SH3 domain structure. Recruits cytoplasmic proteins through SH2-phospho-tyrosine interaction. Phosphorylated by Abl, IGF-IR and EGFR. Phosphorylation induces a change in intramolecular folding and SH2 interactions, causing its rapid dissociation from the tyrosine kinase complex.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis; Oncoprotein

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: cytoplasm; plasma membrane; cytosol; nucleus; actin cytoskeleton

Molecular Function: protein phosphorylated amino acid binding; protein binding; SH3/SH2 adaptor activity; ephrin receptor binding; SH2 domain binding

Biological Process: regulation of transcription from RNA polymerase II promoter; platelet activation; regulation of Rac protein signal transduction; nerve growth factor receptor signaling pathway; activation of MAPKK activity; regulation of actin cytoskeleton organization and biogenesis; ephrin receptor signaling pathway; insulin receptor signaling pathway; innate immune response; vascular endothelial growth factor receptor signaling pathway; blood coagulation