Typical Testing Data/Standard Curve (for reference only)
Human ADAM10 ELISA Kit | ADAM10 elisa kit
Human ADAM10 (A Disintegrin And Metalloprotease 10) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ACV-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACV-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACV-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ACV-A. You can calculate the concentration of Human ACV-A in the samples by comparing the OD of the samples to the standard curve!!Background/Introduction: Activin A is a disulphide-linked homodimer of the b-subunit of inhibin, a member of the transforming growth factor-b (TGF-b) 1 superfamily of proteins [1]. Although four activin genes have been discovered in humans, most studies have focused on the cell biology of activin A. This molecule is involved in the regulation of mitogenesis, embryogenesis, apoptosis, wound repair and other regulatory processes in vivo. Activin A has also been shown to play important roles in the pluripotency, proliferation and differentiation of embryonic stem cells, underpinning an emerging area of biomedical research. Activin A is initially produced as a large precursor molecule, with the pro-region separated from the mature region by a polybasic motif [1]. This motif is putatively cleaved in mammalian cells by subtilisin-like pro-protein convertases (SPCs), such as furin to produce the mature protein. Activin A has been purified from several sources, including porcine follicular fluid and ovine amniotic fluid [2].
NCBI and Uniprot Product Information
NCBI Description
Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. [provided by RefSeq, Jul 2008]
Uniprot Description
ADAM10: Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth- like factor, ephrin-A2 and for constitutive and regulated alpha- secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.
Protein type: Cell adhesion; Cell surface; Membrane protein, integral; Motility/polarity/chemotaxis; EC 3.4.24.81; Protease; Vesicle
Chromosomal Location of Human Ortholog: 15q22
Cellular Component: Golgi apparatus; focal adhesion; cell surface; membrane; intracellular membrane-bound organelle; cytoplasm; postsynaptic density; integral to membrane; plasma membrane; Golgi-associated vesicle; nucleus
Molecular Function: integrin binding; protein binding; protein homodimerization activity; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; endopeptidase activity; protein kinase binding; SH3 domain binding; receptor binding
Biological Process: integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; axon guidance; extracellular matrix organization and biogenesis; Notch signaling pathway; in utero embryonic development; constitutive protein ectodomain proteolysis; membrane protein ectodomain proteolysis; Notch receptor processing; negative regulation of cell adhesion; protein amino acid phosphorylation; positive regulation of cell growth; extracellular matrix disassembly; collagen catabolic process; PMA-inducible membrane protein ectodomain proteolysis; cell-cell signaling; monocyte activation; positive regulation of cell proliferation; ephrin receptor signaling pathway; positive regulation of cell migration
Disease: Reticulate Acropigmentation Of Kitamura; Alzheimer Disease 18
Research Articles on ADAM10
Similar Products
Product Notes
The Human ADAM10 adam10 (Catalog #AAA2510853) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2510853 ELISA Kit recognizes Human ADAM10. It is sometimes possible for the material contained within the vial of "ADAM10, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
