Human ADAM metallopeptidase with thrombospondin type 1 motif4, ADAMTS4 ELISA Kit | ADAMTS4 elisa kit

Human ADAM metallopeptidase with thrombospondin type 1 motif4, ADAMTS4 ELISA Kit

Cat. Num. AAA9302507

• Gene Names: ADAMTS4; ADMP-1; ADAMTS-2; ADAMTS-4
• Reactivity: Human
• Synonyms: ADAM metallopeptidase with thrombospondin type 1 motif4; ADAMTS4; Human ADAM metallopeptidase with thrombospondin type 1 motif4; ADAMTS4 ELISA Kit; ADAMTS4 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human ADAMTS4 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-1000pg/ml.
• Sensitivity: 5.0pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated ADAMTS4 concentrations in Human serum, plasma and other body fluids. Using Purified Human ADAMTS4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ADAMTS4 and ADAMTS4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ADAMTS4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for ADAMTS4 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated ADAMTS4 concentrations in Human serum, plasma and other body fluids. Using Purified Human ADAMTS4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ADAMTS4 and ADAMTS4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ADAMTS4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 157427675
• NCBI GeneID: 9507
• NCBI Accession #: NP_005090.3
• NCBI GenBank Nucleotide #: NM_005099.4
• UniProt Accession #: O75173; Q5VTW2; Q6P4Q8; Q6UWA8; Q9UN83
• Molecular Weight: 90,197 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 4 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif, 4
• NCBI Official Symbol: ADAMTS4
• NCBI Official Synonym Symbols: ADMP-1; ADAMTS-2; ADAMTS-4
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAM-TS4; ADAM-TS 4; aggrecanase-1; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 4
• Protein Family: A disintegrin and metalloproteinase with thrombospondin motifs
• UniProt Gene Name: ADAMTS4
• UniProt Synonym Gene Names: KIAA0688; ADAM-TS 4; ADAM-TS4; ADAMTS-4
• UniProt Entry Name: ATS4_HUMAN

NCBI Description

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAMTS4: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-|-Ala-393' site.

Protein type: Protease; Secreted; EC 3.4.24.82; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q21-q23

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: peptidase activity; protein binding; protease binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; defense response to bacterium; proteolysis; skeletal development