Typical Testing Data/Standard Curve (for reference only)
Human Acyl Coenzyme A Synthetase Short Chain Family, Member 2 (ACSS2) ELISA Kit | ACSS2 elisa kit
Human Acyl Coenzyme A Synthetase Short Chain Family, Member 2 (ACSS2)(Sandwich ELISA) ELISA Kit
Gene Names
ACSS2; ACS; ACSA; ACAS2; ACECS; dJ1161H23.1
Reactivity
Human
Applications
ELISA
Synonyms
Acyl Coenzyme A Synthetase Short Chain Family; Member 2 (ACSS2); Human Acyl Coenzyme A Synthetase Short Chain Family; Member 2 (ACSS2)(Sandwich ELISA) ELISA Kit; ELISA Kit for Acyl Coenzyme A Synthetase Short Chain Family; ACSS2/Acyl Coenzyme A Synthetase Short Chain Family; Member 2; ACS; ACAS2; ACSA; AceCS; MYH7B; Acetyl-Coenzyme A Synthetase 2(ADP Forming); Acyl-activating enzyme; ACSS2 elisa kit
Reactivity
Human
Specificity
Reactive with Human Acyl Coenzyme A Synthetase Short Chain Family, Member 2 (ACSS2)
This assay has high sensitivity and excellent specificity for detection of Acyl Coenzyme A Synthetase Short Chain Family, Member 2 (ACSS2). No significant cross-reactivity or interference between Acyl Coenzyme A Synthetase Short Chain Family, Member 2 (ACSS2) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Acyl Coenzyme A Synthetase Short Chain Family, Member 2 (ACSS2). No significant cross-reactivity or interference between Acyl Coenzyme A Synthetase Short Chain Family, Member 2 (ACSS2) and analogues was observed.
Applicable Applications for ACSS2 elisa kit
ELISA (EIA)
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Assay Type
Sandwich ELISA
Sample Types
tissue homogenates, cell lysates and other biological fluids
Detection Range
78-5,000pg/mL
Sensitivity
29pg/mL
Detection Method
Double-antibody Sandwich
Assay Length
3 hours
Assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acyl Coenzyme A Synthetase Short Chain Family, Member 2 (ACSS2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acyl Coenzyme A Synthetase Short Chain Family, Member 2 (ACSS2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100.
Intra-Assay: CV<10%.
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acyl Coenzyme A Synthetase Short Chain Family, Member 2 (ACSS2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100.
Intra-Assay: CV<10%.
Inter-Assay: CV<12%
Test Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Lactoperoxidase (LPO). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Lactoperoxidase (LPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Lactoperoxidase (LPO) level in the sample or standard.
Assay Procedure Summary
1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 50ul Stop Solution. Read at 450nm immediately.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 50ul Stop Solution. Read at 450nm immediately.
Preparation and Storage
Store at 4 degree C for 1 month; -20 degree C for long-term (within shelf life)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Product Categories/Family for ACSS2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
78,580 Da
NCBI Official Full Name
acetyl-coenzyme A synthetase, cytoplasmic isoform 2
NCBI Official Synonym Full Names
acyl-CoA synthetase short-chain family member 2
NCBI Official Symbol
ACSS2
NCBI Official Synonym Symbols
ACS; ACSA; ACAS2; ACECS; dJ1161H23.1
NCBI Protein Information
acetyl-coenzyme A synthetase, cytoplasmic; acetate thiokinase; acetate-CoA ligase; acyl-activating enzyme; cytoplasmic acetyl-coenzyme A synthetase; acetyl-Coenzyme A synthetase 2 (ADP forming)
UniProt Protein Name
Acetyl-coenzyme A synthetase, cytoplasmic
Protein Family
UniProt Gene Name
ACSS2
UniProt Synonym Gene Names
ACAS2; ACS; AceCS
UniProt Entry Name
ACSA_HUMAN
NCBI Description
This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]
Uniprot Description
ACSS2: Activates acetate so that it can be used for lipid synthesis or for energy generation. Monomer. Belongs to the ATP-dependent AMP-binding enzyme family.
Protein type: Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Ligase; EC 6.2.1.1; Carbohydrate Metabolism - pyruvate
Chromosomal Location of Human Ortholog: 20q11.22
Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; cytosol
Molecular Function: acetate-CoA ligase activity; ATP binding; AMP binding
Biological Process: acetyl-CoA biosynthetic process from acetate; xenobiotic metabolic process; lipid biosynthetic process; ethanol oxidation; propionate biosynthetic process; acetate biosynthetic process
Research Articles on ACSS2
Similar Products
Product Notes
The Human ACSS2 acss2 (Catalog #AAA2125277) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2125277 ELISA Kit recognizes Human ACSS2. AAA Biotech's Acyl Coenzyme A Synthetase Short Chain Family, Member 2 (ACSS2) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the ACSS2 acss2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Acyl Coenzyme A Synthetase Short Chain Family, Member 2 (ACSS2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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