Human Acyl Coenzyme A Dehydrogenase, Long Chain (ACADL) ELISA Kit | ACADL elisa kit

Human Acyl Coenzyme A Dehydrogenase, Long Chain (ACADL) ELISA Kit

Cat. Num. AAA2707639

• Gene Names: ACADL; LCAD; ACAD4
• Reactivity: Human
• Synonyms: Acyl Coenzyme A Dehydrogenase; Long Chain (ACADL); Human Acyl Coenzyme A Dehydrogenase; Long Chain (ACADL) ELISA Kit; LCAD; ACAD4; Long-chain specific acyl-CoA dehydrogenase; mitochondrial; ACADL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Acyl Coenzyme A Dehydrogenase, Long Chain (ACADL).; No significant cross-reactivity or interference between Acyl Coenzyme A Dehydrogenase, Long Chain (ACADL) and analogues was observed.
• Sequence Length: 430

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.056ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acyl Coenzyme A Dehydrogenase, Long Chain (ACADL) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acyl Coenzyme A Dehydrogenase, Long Chain (ACADL) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACADL elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acyl Coenzyme A Dehydrogenase, Long Chain (ACADL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acyl Coenzyme A Dehydrogenase, Long Chain (ACADL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acyl Coenzyme A Dehydrogenase, Long Chain (ACADL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acyl Coenzyme A Dehydrogenase, Long Chain (ACADL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 223590148
• NCBI GeneID: 33
• UniProt Accession #: P28330
• NCBI Official Full Name: Long-chain specific acyl-CoA dehydrogenase, mitochondrial
• NCBI Official Synonym Full Names: acyl-CoA dehydrogenase long chain
• NCBI Official Symbol: ACADL
• NCBI Official Synonym Symbols: LCAD; ACAD4
• NCBI Protein Information: long-chain specific acyl-CoA dehydrogenase, mitochondrial
• UniProt Protein Name: Long-chain specific acyl-CoA dehydrogenase, mitochondrial
• Protein Family: Long-chain specific acyl-CoA dehydrogenase
• UniProt Gene Name: ACADL
• UniProt Synonym Gene Names: LCAD
• UniProt Entry Name: ACADL_HUMAN

NCBI Description

The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family, which is a family of mitochondrial flavoenzymes involved in fatty acid and branched chain amino-acid metabolism. This protein is one of the four enzymes that catalyze the initial step of mitochondrial beta-oxidation of straight-chain fatty acid. Defects in this gene are the cause of long-chain acyl-CoA dehydrogenase (LCAD) deficiency, leading to nonketotic hypoglycemia. [provided by RefSeq, Jul 2008]

Uniprot Description

ACADL: Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD). An inborn error of mitochondrial fatty acid beta-oxidation which leads to impaired long-chain fatty acid beta-oxidation. It is clinically heterogeneous, with three major phenotypes: a severe childhood form characterized by early onset, high mortality and high incidence of cardiomyopathy; a milder childhood form with later onset, characterized by hypoketotic hypoglycemia, low mortality and rare cardiomyopathy; an adult form, with isolated skeletal muscle involvement, rhabdomyolysis and myoglobinuria, usually triggered by exercise or fasting. Belongs to the acyl-CoA dehydrogenase family.

Protein type: Mitochondrial; Lipid Metabolism - fatty acid; EC 1.3.8.8; Oxidoreductase

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial membrane; cytoplasm

Molecular Function: palmitoyl-CoA oxidase activity; acyl-CoA dehydrogenase activity; FAD binding; acyl-CoA binding; long-chain-acyl-CoA dehydrogenase activity

Biological Process: carnitine metabolic process, CoA-linked; fatty acid beta-oxidation; cellular lipid catabolic process; carnitine catabolic process; thermoregulation; cellular lipid metabolic process; fatty acid beta-oxidation using acyl-CoA dehydrogenase; negative regulation of fatty acid oxidation; negative regulation of fatty acid biosynthetic process; long-chain fatty acid catabolic process; protein homotetramerization