Human Actinin Alpha 1 (ACTN1) ELISA Kit | ACTN1 elisa kit

Human Actinin Alpha 1 (ACTN1) ELISA Kit

Cat. Num. AAA2704302

• Gene Names: ACTN1; BDPLT15
• Reactivity: Human
• Synonyms: Actinin Alpha 1 (ACTN1); Human Actinin Alpha 1 (ACTN1) ELISA Kit; Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1; ACTN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Actinin Alpha 1 (ACTN1).; No significant cross-reactivity or interference between Actinin Alpha 1 (ACTN1) and analogues was observed.
• Sequence Length: 930

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.115ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Actinin Alpha 1 (ACTN1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Actinin Alpha 1 (ACTN1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACTN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actinin Alpha 1 (ACTN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actinin Alpha 1 (ACTN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actinin Alpha 1 (ACTN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Actinin Alpha 1 (ACTN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 212293358
• NCBI GeneID: 87
• Molecular Weight: 107,141 Da
• NCBI Official Full Name: actinin alpha 1 isoform 3
• NCBI Official Synonym Full Names: actinin alpha 1
• NCBI Official Symbol: ACTN1
• NCBI Official Synonym Symbols: BDPLT15
• NCBI Protein Information: alpha-actinin-1
• UniProt Protein Name: Alpha-actinin-1
• Protein Family: Alpha-actinin
• UniProt Gene Name: ACTN1

NCBI Description

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ACTN1: a cytoskeletal protein of the spectrin superfamily. An actin-binding protein with multiple roles in different cell types. A nonmuscle isoform structurally similar to the erythroid beta spectrin gene.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q24.1|14q22-q24

Cellular Component: actin filament; brush border; cell projection; cytoplasm; cytosol; extracellular exosome; extracellular region; extracellular space; fascia adherens; focal adhesion; intercellular junction; intracellular; plasma membrane; pseudopodium; ruffle; stress fiber; Z disc

Molecular Function: actin filament binding; calcium ion binding; double-stranded RNA binding; integrin binding; ion channel binding; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; protein homodimerization activity; vinculin binding

Biological Process: actin crosslink formation; actin filament bundle formation; actin filament network formation; actin filament organization; focal adhesion assembly; negative regulation of cell motility; platelet activation; platelet degranulation; platelet formation; regulation of apoptosis

Disease: Bleeding Disorder, Platelet-type, 15