Human Acrosin (ACR) ELISA Kit | ACR elisa kit

Human Acrosin (ACR) ELISA Kit

Cat. Num. AAA2703936

• Reactivity: Human
• Synonyms: Acrosin (ACR); Human Acrosin (ACR) ELISA Kit; ACRS; Preproacrosin; ACR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Acrosin (ACR).; No significant cross-reactivity or interference between Acrosin (ACR) and analogues was observed.
• Sequence Length: 421

• Assay Type: Double-antibody Sandwich
• Samples: Sperm
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.062ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acrosin (ACR) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acrosin (ACR) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACR elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acrosin (ACR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acrosin (ACR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acrosin (ACR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acrosin (ACR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 148613878
• NCBI GeneID: 49
• NCBI Accession #: NP_001088.2
• NCBI GenBank Nucleotide #: NM_001097.2
• UniProt Accession #: P10323; Q6ICK2
• Molecular Weight: 45,847 Da
• NCBI Official Full Name: acrosin
• NCBI Official Synonym Full Names: acrosin
• NCBI Official Symbol: ACR
• NCBI Protein Information: acrosin
• UniProt Protein Name: Acrosin
• Protein Family: Acrosin
• UniProt Gene Name: ACR
• UniProt Synonym Gene Names: ACRS

NCBI Description

Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. [provided by RefSeq, Jul 2008]

Uniprot Description

Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.