Human A Disintegrin And Metalloprotease 17 (ADAM17) ELISA Kit | ADAM17 elisa kit

Human A Disintegrin And Metalloprotease 17 (ADAM17) ELISA Kit

Cat. Num. AAA2703428

• Reactivity: Human
• Synonyms: A Disintegrin And Metalloprotease 17 (ADAM17); Human A Disintegrin And Metalloprotease 17 (ADAM17) ELISA Kit; CD156b; TACE; CSVP; Tumor Necrosis Factor; Alpha; Converting Enzyme; Disintegrin and metalloproteinase domain-containing protein 17; Snake venom-like protease; ADAM17 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 17 (ADAM17).; No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 17 (ADAM17) and analogues was observed.
• Sequence Length: 1095

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 17 (ADAM17) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 17 (ADAM17) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAM17 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 17 (ADAM17). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 17 (ADAM17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 17 (ADAM17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloprotease 17 (ADAM17) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 110611170
• NCBI GeneID: 170691
• NCBI Accession #: NP_620688.2
• NCBI GenBank Nucleotide #: NM_139057.3
• UniProt Accession #: Q8TE56; Q2I7G4; Q6ZN75
• Molecular Weight: 54,757 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 17 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif 17
• NCBI Official Symbol: ADAMTS17
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 17
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 17
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAMTS17
• UniProt Synonym Gene Names: ADAM-TS 17; ADAM-TS17; ADAMTS-17

NCBI Description

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. ADAMTS family members share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature protein, which may promote breast cancer cell growth and survival. Mutations in this gene are associated with a Weill-Marchesani-like syndrome, which is characterized by lenticular myopia, ectopia lentis, glaucoma, spherophakia, and short stature. [provided by RefSeq, May 2016]

Uniprot Description

ADAMTS17: Defects in ADAMTS17 are the cause of Weill-Marchesani- like syndrome (WMLS). It is a disorder characterized by many of the key features of Weill-Marchesani syndrome, including lenticular myopia, ectopia lentis, glaucoma, spherophakia and short stature. However, the characteristic brachydactyly or decreased joint flexibility of Weill-Marchesani syndrome are absent. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.-; Motility/polarity/chemotaxis; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: proteinaceous extracellular matrix

Molecular Function: metalloendopeptidase activity; nucleic acid binding; zinc ion binding

Biological Process: proteolysis

Disease: Weill-marchesani-like Syndrome