Typical Testing Data/Standard Curve (for reference only)
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Human 5-MTHF (5-Methyltetrahydrofolate) ELISA Kit | 5-MTHF elisa kit
Human 5-MTHF (5-Methyltetrahydrofolate) ELISA Kit
Reactivity
Human
Synonyms
5-MTHF (5-Methyltetrahydrofolate); Human 5-MTHF (5-Methyltetrahydrofolate) ELISA Kit; 5-MTHF elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of 5-MTHF. No significant cross-reactivity or interference between 5-MTHF and analogues was observed.
Sequence Length
208
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Competitive
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Intra-assay Precision
CV<8%
Inter-assay Precision
CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for 5-MTHF elisa kit
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
23,092 Da
NCBI Official Full Name
5-methyltetrahydrofolate, partial
UniProt Protein Name
5-methyltetrahydrofolate
UniProt Gene Name
OBRU01_06352
Similar Products
Product Notes
The Human 5-MTHF obru01_06352 (Catalog #AAA7606302) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7606302 ELISA Kit recognizes Human 5-MTHF. It is sometimes possible for the material contained within the vial of "5-MTHF (5-Methyltetrahydrofolate), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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