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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human 28S ribosomal protein S23, mitochondrial (MRPS23) Standard Curve.)

Human 28S Ribosomal Protein S23, Mitochondrial (MRPS23) ELISA Kit | MRPS23 elisa kit

Human 28S Ribosomal Protein S23, Mitochondrial (MRPS23) ELISA Kit

Gene Names
MRPS23; CGI-138; HSPC329; MRP-S23
Reactivity
Human
Synonyms
28S Ribosomal Protein S23; Mitochondrial (MRPS23); Human 28S Ribosomal Protein S23; Mitochondrial (MRPS23) ELISA Kit; MRPS23; CGI-138; HSPC329; MRP-S23; MRPS23 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human 28S ribosomal protein S23, mitochondrial (MRPS23) ELISA Kit has high sensitivity and excellent specificity for detection of Human MRPS23. No significant cross-reactivity or interference between Human MRPS23 and analogues was observed.
Sequence Length
190
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human 28S ribosomal protein S23, mitochondrial (MRPS23) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human 28S ribosomal protein S23, mitochondrial (MRPS23) Standard Curve.)
Related Product Information for MRPS23 elisa kit
Description: This Human 28S ribosomal protein S23, mitochondrial (MRPS23) ELISA Kit employs a two-site sandwich ELISA to quantitate MRPS23 in samples. An antibody specific for MRPS23 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MRPS23 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRPS23 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRPS23 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS23 encodes a 28S subunit protein, mitochondrial ribosomal protein S23. A pseudogene corresponding to MRPS23 is found on chromosome 7p.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
28S ribosomal protein S23, mitochondrial
NCBI Official Synonym Full Names
mitochondrial ribosomal protein S23
NCBI Official Symbol
MRPS23
NCBI Official Synonym Symbols
CGI-138; HSPC329; MRP-S23
NCBI Protein Information
28S ribosomal protein S23, mitochondrial
UniProt Protein Name
28S ribosomal protein S23, mitochondrial
Protein Family
UniProt Gene Name
MRPS23
UniProt Synonym Gene Names
MRP-S23; S23mt
UniProt Entry Name
RT23_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 7p. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPS23: Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.

Protein type: Mitochondrial; Ribosomal; Translation

Chromosomal Location of Human Ortholog: 17q22-q23

Cellular Component: intermediate filament cytoskeleton; mitochondrial inner membrane; mitochondrion; nuclear membrane

Molecular Function: protein binding

Research Articles on MRPS23

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Product Notes

The Human MRPS23 mrps23 (Catalog #AAA9715197) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715197 ELISA Kit recognizes Human MRPS23. It is sometimes possible for the material contained within the vial of "28S Ribosomal Protein S23, Mitochondrial (MRPS23), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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