Human 28S ribosomal protein S14, mitochondrial (MRPS14) ELISA Kit | MRPS14 elisa kit

Human 28S ribosomal protein S14, mitochondrial (MRPS14) ELISA Kit

Cat. Num. AAA7239130

• Gene Names: MRPS14; S14mt; MRP-S14; HSMRPS14; DJ262D12.2
• Reactivity: Human
• Synonyms: 28S ribosomal protein S14; mitochondrial (MRPS14); Human 28S ribosomal protein S14; mitochondrial (MRPS14) ELISA Kit; MRPS14 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MRPS14. No significant cross-reactivity or interference between MRPS14 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MRPS14 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MRPS14 elisa kit

Intended Uses: This MRPS14 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanMRPS14. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: MRPS14 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MRPS14 antibody and an MRPS14-HRP conjugate. The assay sample and buffer are incubated together with MRPS14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MRPS14 concentration since MRPS14 from samples and MRPS14-HRP conjugate compete for the anti-MRPS14 antibody binding site. Since the number of sites is limited, as more sites are occupied by MRPS14 from the sample, fewer sites are left to bind MRPS14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MRPS14 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 11559927
• NCBI GeneID: 63931
• NCBI Accession #: NP_071383.1
• NCBI GenBank Nucleotide #: NM_022100.2
• Molecular Weight: 15,139 Da
• NCBI Official Full Name: 28S ribosomal protein S14, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein S14
• NCBI Official Symbol: MRPS14
• NCBI Official Synonym Symbols: S14mt; MRP-S14; HSMRPS14; DJ262D12.2
• NCBI Protein Information: 28S ribosomal protein S14, mitochondrial; mitochondrial 28S ribosomal protein S14
• UniProt Protein Name: 28S ribosomal protein S14, mitochondrial
• Protein Family: 28S ribosomal protein
• UniProt Gene Name: MRPS14
• UniProt Synonym Gene Names: MRP-S14; S14mt
• UniProt Entry Name: RT14_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S14P family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]

Uniprot Description

MRPS14: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S14P family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]

Protein type: Mitochondrial; Ribosomal

Chromosomal Location of Human Ortholog: 1q25.1

Cellular Component: nuclear membrane; mitochondrion; mitochondrial small ribosomal subunit; mitochondrial inner membrane; mitochondrial ribosome

Molecular Function: structural constituent of ribosome

Biological Process: mitochondrial translation; translation; organelle organization and biogenesis