Typical Testing Data/Standard Curve (for reference only)
Human 1 phosphatidylinositol 3 phosphate 5 kinase (PIP5K3) ELISA Kit | PIKFYVE elisa kit
Human 1 phosphatidylinositol 3 phosphate 5 kinase (PIP5K3) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: P1P5K3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-P1P5K3 antibody and an P1P5K3-HRP conjugate. The assay sample and buffer are incubated together with P1P5K3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the P1P5K3 concentration since P1P5K3 from samples and P1P5K3-HRP conjugate compete for the anti-P1P5K3 antibody binding site. Since the number of sites is limited, as more sites are occupied by P1P5K3 from the sample, fewer sites are left to bind P1P5K3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P1P5K3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome carrier vesicles from early endosomes. Mutations in this gene cause corneal fleck dystrophy (CFD); an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. Histologically, these flecks appear to be keratocytes distended with lipid and mucopolysaccharide filled intracytoplasmic vacuoles. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, May 2010]
Uniprot Description
PIKFYVE: The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo- inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. Plays a role in the biogenesis of endosome carrier vesicles (ECV)/ multivesicular bodies (MVB) transport intermediates from early endosomes. Defects in PIKFYVE are the cause of corneal fleck dystrophy (CFD). CFD is an autosomal dominant disorder of the cornea characterized by numerous small white flecks scattered in all levels of the stroma. Although CFD may occasionally cause mild photophobia, patients are typically asymptomatic and have normal vision. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Carbohydrate Metabolism - inositol phosphate; Motility/polarity/chemotaxis; EC 2.7.1.150; Kinase, lipid
Chromosomal Location of Human Ortholog: 2q34
Cellular Component: Golgi membrane; late endosome membrane; perinuclear region of cytoplasm; early endosome membrane; endosome membrane; cytoplasmic vesicle; intercellular junction; cytosol; lipid raft
Molecular Function: protein binding; 1-phosphatidylinositol-3-phosphate 5-kinase activity; 1-phosphatidylinositol-4-phosphate 5-kinase activity; phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity; metal ion binding; ATP binding
Biological Process: receptor-mediated endocytosis; myelin formation; cellular protein metabolic process; phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process; retrograde transport, endosome to Golgi
Disease: Corneal Dystrophy, Fleck
Research Articles on PIKFYVE
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Product Notes
The Human PIKFYVE pikfyve (Catalog #AAA7203853) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7203853 ELISA Kit recognizes Human PIKFYVE. It is sometimes possible for the material contained within the vial of "1 phosphatidylinositol 3 phosphate 5 kinase (PIP5K3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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