Human S100 Calcium Binding Protein B (S100B) DIY ELISA Kit | S100B diy elisa kit

Human S100 Calcium Binding Protein B (S100B) ELISA Kit DIY Materials

Cat. Num. AAA2088665

• Gene Names: S100B; NEF; S100; S100-B; S100beta
• Reactivity: Human
• Synonyms: S100 Calcium Binding Protein B (S100B); Human S100 Calcium Binding Protein B (S100B) ELISA Kit DIY Materials; S100-B; NEF; S100Beta; S-100 protein subunit beta; S100B diy elisa kit

• Reactivity: Human
• Specificity: The Antibody Pairs in the kit have high sensitivity and excellent specificity for detection of S100A4.
• Sequence Length: 92

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Sandwich
• Detection Range: 0.78-50ng/mL.
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100B diy elisa kit

Intended Uses: This 'Do it Yourself (DIY)' assay kit contains materials for developing your own immunoassays. This may include the development of sandwich ELISA to measure S100A4 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids in vitro. This kit contains sufficient materials for preparation of at least five 96-well plates. Sample Preparation, Assay Protocol and Detection Range for Reference are provided as suggestions only. Researchers should optimize the use of kit materials and protocols for their own model system and determine if the kit is suitable for their samples and immunoassay.

Product Categories/Family for S100B diy elisa kit

Tumor immunity; Infection immunity; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 12804681
• NCBI GeneID: 6285
• Molecular Weight: 10,713 Da
• NCBI Official Full Name: S100 calcium binding protein B
• NCBI Official Synonym Full Names: S100 calcium binding protein B
• NCBI Official Symbol: S100B
• NCBI Official Synonym Symbols: NEF; S100; S100-B; S100beta
• NCBI Protein Information: protein S100-B
• UniProt Protein Name: Protein S100-B
• Protein Family: Protein
• UniProt Gene Name: S100B

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

Uniprot Description

S100B: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with AGER. The S100B dimer interacts with two molecules of CAPZA1. Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. Belongs to the S-101 family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cytoplasm; extracellular region; intracellular membrane-bound organelle; nucleus; perinuclear region of cytoplasm; ruffle

Molecular Function: calcium-dependent protein binding; identical protein binding; protein binding; protein homodimerization activity; RAGE receptor binding; tau protein binding; zinc ion binding

Biological Process: axonogenesis; cell proliferation; central nervous system development; innate immune response; learning and/or memory; positive regulation of I-kappaB kinase/NF-kappaB cascade