Human Pancreatic Polypeptide (PP) DIY ELISA Kit | PP diy elisa kit

Human Pancreatic Polypeptide (PP) ELISA Kit DIY Materials

Cat. Num. AAA2089067

• Gene Names: PPY; PP; PNP
• Reactivity: Human
• Synonyms: Pancreatic Polypeptide (PP); Human Pancreatic Polypeptide (PP) ELISA Kit DIY Materials; PPY; PNP; Pancreatic Polypeptide Y; Obinepitide; Pancreatic icosapeptide; Pancreatic prohormone; PP diy elisa kit

• Reactivity: Human
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Polypeptide (PP). No significant cross-reactivity or interference between Polypeptide (PP) and analogues was observed.
• Sequence Length: 95

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Competitive Inhibition ELISA for Antigen Detection
• Samples: Serum, plasma and other biological fluids.
• Detection Range: 37.0-3,000pg/mL
• Sensitivity: 14.2pg/mL
• Reagent Contents: Capture Antibody, Biontin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 2. (Catalog MBS2089472)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for PP diy elisa kit

Endocrinology

NCBI and Uniprot Product Information

• NCBI GI #: 119572034
• NCBI GeneID: 5539
• Molecular Weight: 18,786 Da
• NCBI Official Full Name: pancreatic polypeptide
• NCBI Official Synonym Full Names: pancreatic polypeptide
• NCBI Official Symbol: PPY
• NCBI Official Synonym Symbols: PP; PNP
• NCBI Protein Information: pancreatic prohormone
• UniProt Protein Name: Pancreatic prohormone
• UniProt Gene Name: PPY
• UniProt Synonym Gene Names: PNP; PP; PH; PI

NCBI Description

This gene encodes a member of the neuropeptide Y (NPY) family of peptides. The encoded 95 aa preproprotein is synthesized in the pancreatic islets of Langerhans and proteolytically processed to generate two peptide products. These products include the active pancreatic hormone of 36 aa and an icosapeptide of unknown function. This hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

PPY: Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions. Belongs to the NPY family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: extracellular region; extracellular space

Molecular Function: G-protein-coupled receptor binding; hormone activity; neuropeptide hormone activity; receptor binding

Biological Process: cell-cell signaling; digestion; feeding behavior; neuropeptide signaling pathway; protein secretion; regulation of appetite