Human Interleukin 28A (IL28A) DIY ELISA Kit | IL28A diy elisa kit

Human Interleukin 28A (IL28A) ELISA Kit DIY Materials

Cat. Num. AAA2089137

• Gene Names: IFNL2; IL28A; IL-28A
• Reactivity: Human
• Synonyms: Interleukin 28A (IL28A); Human Interleukin 28A (IL28A) ELISA Kit DIY Materials; IFNL2; Interferon; Lambda 2; Cytokine Zcyto20; IL28A diy elisa kit

• Reactivity: Human
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 28A (IL28A). No significant cross-reactivity or interference between Interleukin 28A (IL28A) and analogues was observed.
• Sequence Length: 200

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 7.8-500pg/mL
• Sensitivity: 3.0pg/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for IL28A diy elisa kit

Cytokine; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 26024321
• NCBI GeneID: 282616
• NCBI Accession #: NP_742150.1
• NCBI GenBank Nucleotide #: NM_172138.1
• UniProt Accession #: Q8IZJ0; Q45KQ8; Q6VN55; Q8IWL7
• Molecular Weight: 22,288 Da
• NCBI Official Full Name: interferon lambda-2
• NCBI Official Synonym Full Names: interferon lambda 2
• NCBI Official Symbol: IFNL2
• NCBI Official Synonym Symbols: IL28A; IL-28A
• NCBI Protein Information: interferon lambda-2
• UniProt Protein Name: Interferon lambda-2
• UniProt Gene Name: IFNL2
• UniProt Synonym Gene Names: IL28A; ZCYTO20; IFN-lambda-2; IL-28A

NCBI Description

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28B (IL28B), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]

Uniprot Description

IL28A: Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Belongs to the IL-28/IL-29 family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular region; extracellular space

Molecular Function: receptor binding

Biological Process: defense response to virus; innate immune response; mucosal immune response; regulation of T cell activation