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Human Interleukin 18 (IL18) DIY ELISA Kit | IL18 diy elisa kit

Human Interleukin 18 (IL18) ELISA Kit DIY Materials

Gene Names
IL18; IGIF; IL-18; IL-1g; IL1F4
Reactivity
Human
Synonyms
Interleukin 18 (IL18); Human Interleukin 18 (IL18) ELISA Kit DIY Materials; IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma; IL18 diy elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 18 (IL18). No significant cross-reactivity or interference between Interleukin 18 (IL18) and analogues was observed.
Sequence Length
193
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
15.6-1000pg/mL
Sensitivity
5.9pg/mL
Reagent Contents
Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Product Categories/Family for IL18 diy elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,896 Da
NCBI Official Full Name
interleukin-18 isoform 1
NCBI Official Synonym Full Names
interleukin 18
NCBI Official Symbol
IL18
NCBI Official Synonym Symbols
IGIF; IL-18; IL-1g; IL1F4
NCBI Protein Information
interleukin-18
UniProt Protein Name
Interleukin-18
Protein Family
UniProt Gene Name
IL18
UniProt Synonym Gene Names
IGIF; IL1F4; IL-18; IFN-gamma-inducing factor; IL-1 gamma

NCBI Description

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

Uniprot Description

IL18: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Belongs to the IL-1 family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: cytosol; extracellular region; extracellular space

Molecular Function: cytokine activity; protein binding

Biological Process: activation of protein kinase B; angiogenesis; cell-cell signaling; chemokine biosynthetic process; cholesterol homeostasis; granulocyte macrophage colony-stimulating factor biosynthetic process; immune response; inflammatory response; interferon-gamma biosynthetic process; interleukin-13 biosynthetic process; interleukin-2 biosynthetic process; lipopolysaccharide-mediated signaling pathway; MAPKKK cascade; positive regulation of activated T cell proliferation; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of inflammatory response; positive regulation of interferon-gamma production; positive regulation of interleukin-17 production; positive regulation of natural killer cell proliferation; positive regulation of NK T cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of smooth muscle cell proliferation; positive regulation of T-helper 2 cell differentiation; positive regulation of tissue remodeling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of cell adhesion; sleep; T-helper 1 type immune response; T-helper 2 type immune response

Research Articles on IL18

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Product Notes

The Human IL18 il18 (Catalog #AAA2088350) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2088350 DIY ELISA Kit recognizes Human IL18. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the IL18 il18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 18 (IL18), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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