Human Adiponectin (ADP) DIY ELISA Kit | ADP diy elisa kit
Human Adiponectin (ADP) ELISA Kit DIY Materials
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
NCBI and Uniprot Product Information
NCBI Description
This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010]
Uniprot Description
adiponectin: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2. Interacts with CTRP9A via the C1q domain (heterotrimeric complex). Synthesized exclusively by adipocytes and secreted into plasma.
Protein type: Endoplasmic reticulum; Hormone; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 3q27.3
Cellular Component: cell surface; endoplasmic reticulum; extracellular region; extracellular space
Molecular Function: hormone activity; identical protein binding; protein binding; protein homodimerization activity; receptor binding; sialic acid binding
Biological Process: brown fat cell differentiation; cellular response to insulin stimulus; fatty acid beta-oxidation; fatty acid oxidation; generation of precursor metabolites and energy; glucose homeostasis; glucose metabolic process; negative regulation of blood pressure; negative regulation of cell migration; negative regulation of fat cell differentiation; negative regulation of gluconeogenesis; negative regulation of granulocyte differentiation; negative regulation of heterotypic cell-cell adhesion; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of inflammatory response; negative regulation of low-density lipoprotein receptor biosynthetic process; negative regulation of macrophage differentiation; negative regulation of MAP kinase activity; negative regulation of phagocytosis; negative regulation of protein amino acid autophosphorylation; negative regulation of smooth muscle cell migration; negative regulation of smooth muscle cell proliferation; negative regulation of synaptic transmission; negative regulation of transcription, DNA-dependent; negative regulation of tumor necrosis factor production; positive regulation of cellular protein metabolic process; positive regulation of fatty acid metabolic process; positive regulation of glucose import; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-8 production; positive regulation of myeloid cell apoptosis; positive regulation of protein amino acid phosphorylation; positive regulation of signal transduction; protein homooligomerization; response to glucose stimulus
Disease: Adiponectin, Serum Level Of, Quantitative Trait Locus 1
Research Articles on ADP
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Product Notes
The Human ADP adipoq (Catalog #AAA2088697) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2088697 DIY ELISA Kit recognizes Human ADP. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the ADP adipoq for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Adiponectin (ADP), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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