Human TLR2 (Toll-Like Receptor 2) CLIA Kit | TLR2 clia kit

Human TLR2 (Toll-Like Receptor 2) CLIA Kit

Cat. Num. AAA2531401

• Reactivity: Human
• Synonyms: TLR2 (Toll-Like Receptor 2); Human TLR2 (Toll-Like Receptor 2) CLIA Kit; CD282; TIL4; TLR2 clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant TLR2. No significant cross-reactivity or interference between TLR2 and analogues was observed.
• Sequence Length: 784

• Assay Type: Sandwich
• Detection Range: 62.5-4000pg/mL
• Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TLR2 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of TLR2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to TLR2. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TLR2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TLR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of TLR2. You can calculate the concentration of TLR2 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27806379
• NCBI GeneID: 281534
• NCBI Accession #: NP_776622.1
• NCBI GenBank Nucleotide #: NM_174197.2
• Molecular Weight: 90,205 Da
• NCBI Official Full Name: toll-like receptor 2
• NCBI Official Symbol: TLR2
• NCBI Protein Information: toll-like receptor 2
• UniProt Protein Name: Toll-like receptor 2
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR2
• UniProt Entry Name: TLR2_BOVIN

Uniprot Description

Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (). May also promote apoptosis in response to lipoproteins. Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2:TLR6:CD14:CD36 in response to diacylated lipopeptides and TLR2:TLR1:CD14 in response to triacylated lipopeptides ().