Human Semaphorin 3A (SEMA3A) CLIA Kit | SEMA3A clia kit

Human Semaphorin 3A (SEMA3A) CLIA Kit

Cat. Num. AAA2000872

• Gene Names: SEMA3A; HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III
• Reactivity: Human
• Synonyms: Semaphorin 3A (SEMA3A); Human Semaphorin 3A (SEMA3A) CLIA Kit; SEMAL; Hsema-I; Hsema-III; SEMA1; SEMAD; SEMAIII; SemD; Coll-1; Sema Domain; Immunoglobulin Domain(Ig); Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A; SEMA3A clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Semaphorin 3A (SEMA3A).; No significant cross-reactivity or interference between Semaphorin 3A (SEMA3A) and analogues was observed.
• Sequence Length: 771

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 13.7-10,000pg/mL
• Sensitivity: < 5.3pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 3A (SEMA3A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 3A (SEMA3A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SEMA3A clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Semaphorin 3A (SEMA3A). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Semaphorin 3A (SEMA3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Semaphorin 3A (SEMA3A) level in the sample or standard.;

Product Categories/Family for SEMA3A clia kit

CD & Adhesion molecule

NCBI and Uniprot Product Information

• NCBI GI #: 5174673
• NCBI GeneID: 10371
• NCBI Accession #: NP_006071.1
• NCBI GenBank Nucleotide #: NM_006080.2
• UniProt Accession #: Q14563
• Molecular Weight: 88,889 Da
• NCBI Official Full Name: semaphorin-3A
• NCBI Official Synonym Full Names: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
• NCBI Official Symbol: SEMA3A
• NCBI Official Synonym Symbols: HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III
• NCBI Protein Information: semaphorin-3A
• UniProt Protein Name: Semaphorin-3A
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA3A
• UniProt Synonym Gene Names: SEMAD; Sema III
• UniProt Entry Name: SEM3A_HUMAN

NCBI Description

This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008]

Uniprot Description

SEMA3A: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. Belongs to the semaphorin family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7p12.1

Cellular Component: membrane; axon; dendrite; extracellular region

Molecular Function: chemorepellent activity; receptor activity; semaphorin receptor binding

Biological Process: trigeminal nerve structural organization; axon guidance; facial nerve structural organization; apoptosis; regulation of heart rate; olfactory bulb development; dendrite morphogenesis; neuron migration; axonal fasciculation; negative chemotaxis; negative regulation of axon extension involved in axon guidance; axon extension involved in axon guidance; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; sympathetic nervous system development; branchiomotor neuron axon guidance; regulation of axon extension involved in axon guidance; nerve development; sensory system development

Disease: Hypogonadotropic Hypogonadism 16 With Or Without Anosmia