Typical Testing Data/Standard Curve (for reference only)
Human RUNX2 (Runt-Related Transcription Factor 2) CLIA Kit | RUNX2 clia kit
Human RUNX2 (Runt-Related Transcription Factor 2) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to RUNX2. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for RUNX2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain RUNX2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of RUNX2. You can calculate the concentration of RUNX2 in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
AML3: Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'- PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation. Interaction with SATB2 results in enhanced DNA binding and transactivation by these transcription factors. Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 and HIPK3. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with KAT6A and KAT6B. Binds to cyclin B1 CCNB1. Interacts with DDX5. Specifically expressed in osteoblasts. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; DNA-binding
Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; nucleus
Molecular Function: protein domain specific binding; DNA binding; histone deacetylase binding; bHLH transcription factor binding; chromatin binding; transcription factor binding; transcription factor activity; ATP binding
Biological Process: embryonic forelimb morphogenesis; transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; cell maturation; BMP signaling pathway; neuron differentiation; positive regulation of cell proliferation; chondrocyte differentiation; hemopoiesis; skeletal development; endochondral ossification; regulation of odontogenesis of dentine-containing teeth; T cell differentiation; ossification; regulation of osteoblast differentiation; transcription, DNA-dependent; regulation of fibroblast growth factor receptor signaling pathway; embryonic cranial skeleton morphogenesis; chondrocyte development; stem cell differentiation; positive regulation of ossification; odontogenesis of dentine-containing teeth; osteoblast development; osteoblast differentiation; regulation of transcription from RNA polymerase II promoter; positive regulation of osteoblast differentiation; positive regulation of chondrocyte differentiation; skeletal morphogenesis; negative regulation of smoothened signaling pathway; positive regulation of transcription from RNA polymerase II promoter; regulation of ossification; negative regulation of transcription, DNA-dependent; osteoblast fate commitment
Research Articles on RUNX2
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Product Notes
The Human RUNX2 runx2 (Catalog #AAA2531726) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2531726 CLIA Kit recognizes Human RUNX2. It is sometimes possible for the material contained within the vial of "RUNX2 (Runt-Related Transcription Factor 2), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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