Human Renin (REN) CLIA Kit | REN clia kit

Human Renin (REN) CLIA Kit

Cat. Num. AAA2708878

• Gene Names: REN; HNFJ2
• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Renin (REN); Human Renin (REN) CLIA Kit; Renin; angiotensinogenase; REN clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Renin (REN).; No significant cross-reactivity or interference between Renin (REN) and analogues was observed.
• Sequence Length: 406

• Applicable Applications for REN clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 5.49-4,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 2.24pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Renin (REN) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Renin (REN) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Renin (REN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Renin (REN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Renin (REN) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 4506475
• NCBI GeneID: 5972
• NCBI Accession #: NP_000528.1
• NCBI GenBank Nucleotide #: NP_000528.1
• UniProt Accession #: P00797
• NCBI Official Full Name: renin preproprotein
• NCBI Official Synonym Full Names: renin
• NCBI Official Symbol: REN
• NCBI Official Synonym Symbols: HNFJ2
• NCBI Protein Information: renin
• UniProt Protein Name: Renin
• Protein Family: Renin
• UniProt Gene Name: REN
• UniProt Entry Name: RENI_HUMAN

NCBI Description

This gene encodes renin, an aspartic protease that is secreted by the kidneys. Renin is a part of the renin-angiotensin-aldosterone system involved in regulation of blood pressure, and electrolyte balance. This enzyme catalyzes the first step in the activation pathway of angiotensinogen by cleaving angiotensinogen to form angiotensin I, which is then converted to angiotensin II by angiotensin I converting enzyme. This cascade can result in aldosterone release, narrowing of blood vessels, and increase in blood pressure as angiotension II is a vasoconstrictive peptide. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause hyperuricemic nephropathy familial juvenile 2, familial hyperproreninemia, and renal tubular dysgenesis. [provided by RefSeq, May 2020]

Uniprot Description

REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.23.15; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular space; cytoplasm; plasma membrane; extracellular region

Molecular Function: peptidase activity; insulin-like growth factor receptor binding; aspartic-type endopeptidase activity; receptor binding

Biological Process: response to cAMP; hormone-mediated signaling; drinking behavior; male gonad development; renin-angiotensin regulation of aldosterone production; cell maturation; response to lipopolysaccharide; angiotensin maturation; proteolysis; cellular protein metabolic process; regulation of blood pressure; regulation of MAPKKK cascade; beta-amyloid metabolic process; mesonephros development; kidney development

Disease: Renal Tubular Dysgenesis; Hyperuricemic Nephropathy, Familial Juvenile, 2