Human Mucin 5 Subtype B (MUC5B) CLIA Kit | MUC5B clia kit

Human Mucin 5 Subtype B (MUC5B) CLIA Kit

Cat. Num. AAA2708826

• Gene Names: MUC5B; MG1; MUC5; MUC9; MUC-5B
• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Mucin 5 Subtype B (MUC5B); Human Mucin 5 Subtype B (MUC5B) CLIA Kit; Mucin 5 Subtype B; MUC5-B; MUC9; MG1; Oligomeric Mucus/Gel-Forming; Mucin 5; Subtype B; Tracheobronchial; Cervical mucin; High molecular weight salivary mucin MG1; Sublingual gland mucin; MUC5B clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mucin 5 Subtype B (MUC5B).; No significant cross-reactivity or interference between Mucin 5 Subtype B (MUC5B) and analogues was observed.
• Sequence Length: 5762

• Applicable Applications for MUC5B clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 54.9-40,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 18.9pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 5 Subtype B (MUC5B) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 5 Subtype B (MUC5B) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Mucin 5 Subtype B (MUC5B). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Mucin 5 Subtype B (MUC5B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Mucin 5 Subtype B (MUC5B) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 301172750
• NCBI GeneID: 727897
• NCBI Accession #: NP_002449.2
• NCBI GenBank Nucleotide #: NP_002449.2
• UniProt Accession #: Q9HC84
• NCBI Official Full Name: mucin-5B
• NCBI Official Synonym Full Names: mucin 5B, oligomeric mucus/gel-forming
• NCBI Official Symbol: MUC5B
• NCBI Official Synonym Symbols: MG1; MUC5; MUC9; MUC-5B
• NCBI Protein Information: mucin-5B
• UniProt Protein Name: Mucin-5B
• Protein Family: Mucin
• UniProt Gene Name: MUC5B
• UniProt Synonym Gene Names: MUC5; MUC-5B
• UniProt Entry Name: MUC5B_HUMAN

NCBI Description

This gene encodes a member of the mucin family of proteins, which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus. This gene has been found to be up-regulated in some human diseases, including sinus mucosa of chronic rhinosinusitis (CRS), CRS with nasal polyposis, chronic obstructive pulmonary disease (COPD) and H. pylori-associated gastric disease, and it may be involved in the pathogenesis of these diseases. [provided by RefSeq, Jul 2010]

Uniprot Description

MUC5B: Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus. Defects in MUC5B are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF). Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease. A common polymorphism in the promoter of MUC5B is associated with familial interstitial pneumonia and idiopathic pulmonary fibrosis, suggesting that dysregulated MUC5B expression in the lung may be involved in the pathogenesis of pulmonary fibrosis (PubMed:21506741).

Protein type: Secreted; Secreted, signal peptide; Cell adhesion

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; Golgi lumen

Molecular Function: protein binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; regulation of macrophage activation; defense response to bacterium; post-translational protein modification

Disease: Pulmonary Fibrosis, Idiopathic