Typical Testing Data/Standard Curve (for reference only)
Human MCP-2/CCL8 (Monocyte Chemotactic Protein 2) CLIA Kit | MCP-2/CCL8 clia kit
Human MCP-2/CCL8 (Monocyte Chemotactic Protein 2) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to MCP-2/CCL8. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MCP-2/CCL8 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MCP-2/CCL8, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of MCP-2/CCL8. You can calculate the concentration of MCP-2/CCL8 in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection. [provided by RefSeq, Sep 2014]
Uniprot Description
CCL8: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Chemokine; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17q11.2
Cellular Component: extracellular space; cell
Molecular Function: heparin binding; CCR chemokine receptor binding; CCR2 chemokine receptor binding; chemokine activity; protein kinase activity; phospholipase activator activity
Biological Process: neutrophil chemotaxis; exocytosis; response to virus; positive regulation of leukocyte migration; signal transduction; chemotaxis; protein amino acid phosphorylation; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; monocyte chemotaxis; cell-cell signaling; calcium ion transport; eosinophil chemotaxis; angiogenesis; inflammatory response; lymphocyte chemotaxis; positive regulation of GTPase activity
Research Articles on MCP-2/CCL8
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Product Notes
The Human MCP-2/CCL8 ccl8 (Catalog #AAA2533051) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533051 CLIA Kit recognizes Human MCP-2/CCL8. It is sometimes possible for the material contained within the vial of "MCP-2/CCL8 (Monocyte Chemotactic Protein 2), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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