Human IGF-1 (Insulin-Like Growth Factor 1) CLIA Kit | IGF-1 clia kit

Human IGF-1 (Insulin-Like Growth Factor 1) CLIA Kit

Cat. Num. AAA2532605

• Gene Names: IGF1; IGF-1; IGF-I
• Reactivity: Human
• Synonyms: IGF-1 (Insulin-Like Growth Factor 1); Human IGF-1 (Insulin-Like Growth Factor 1) CLIA Kit; IGF1; IGFI; IGF-I; IGF1A; IGF-IA; IGF-IB; MGF; Somatomedin C; IGF-1 clia kit

• Reactivity: Human
• Specificity: This kit recognizes Human PSA/KLK3 in samples. No significantcross-reactivity or interference between HumanPSA/KLK3 and analogues was observed.
• Sequence Length: 154

• Samples: Serum, Plasma And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.31-20ng/mL
• Sensitivity: 0.19ng/mL.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGF-1 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of Human PSA/KLK3 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PSA/KLK3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PSA/KLK3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PSA/KLK3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PSA/KLK3. You can calculate the concentration of Human PSA/KLK3 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 118150782
• NCBI GeneID: 281239
• NCBI Accession #: NP_001071296.1
• NCBI GenBank Nucleotide #: NM_001077828.1
• Molecular Weight: 17,066 Da
• NCBI Official Full Name: insulin-like growth factor I preproprotein
• NCBI Official Symbol: IGF1
• NCBI Official Synonym Symbols: IGF-1; IGF-I
• NCBI Protein Information: insulin-like growth factor I
• UniProt Protein Name: Insulin-like growth factor I
• UniProt Gene Name: IGF1
• UniProt Synonym Gene Names: IGF-I
• UniProt Entry Name: IGF1_BOVIN

Uniprot Description

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 ().