Human GSTu2/GSTm2 (Glutathione S Transferase Mu 2, Muscle) CLIA Kit | GSTu2/GSTm2 clia kit

Human GSTu2/GSTm2 (Glutathione S Transferase Mu 2, Muscle) CLIA Kit

Cat. Num. AAA2532909

• Gene Names: GSTM1; MU; H-B; GST1; GTH4; GTM1; MU-1; GSTM1-1; GSTM1a-1a; GSTM1b-1b
• Reactivity: Human
• Synonyms: GSTu2/GSTm2 (Glutathione S Transferase Mu 2; Muscle); Human GSTu2/GSTm2 (Glutathione S Transferase Mu 2; Muscle) CLIA Kit; GSTu2/GSTm2 clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant GSTu2/GSTm2. No significant cross-reactivity or interference between GSTu2/GSTm2 and analogues was observed.
• Sequence Length: 181

• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GSTu2/GSTm2 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of GSTu2/GSTm2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to GSTu2/GSTm2. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GSTu2/GSTm2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GSTu2/GSTm2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of GSTu2/GSTm2. You can calculate the concentration of GSTu2/GSTm2 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 23065547
• NCBI GeneID: 2944
• NCBI Accession #: NP_666533.1
• NCBI GenBank Nucleotide #: NM_146421.2
• Molecular Weight: 21,253 Da
• NCBI Official Full Name: glutathione S-transferase Mu 1 isoform 2
• NCBI Official Synonym Full Names: glutathione S-transferase mu 1
• NCBI Official Symbol: GSTM1
• NCBI Official Synonym Symbols: MU; H-B; GST1; GTH4; GTM1; MU-1; GSTM1-1; GSTM1a-1a; GSTM1b-1b
• NCBI Protein Information: glutathione S-transferase Mu 1
• UniProt Protein Name: Glutathione S-transferase Mu 1
• UniProt Gene Name: GSTM1
• UniProt Synonym Gene Names: GST1
• UniProt Entry Name: GSTM1_HUMAN

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Null mutations of this class mu gene have been linked with an increase in a number of cancers, likely due to an increased susceptibility to environmental toxins and carcinogens. Multiple protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GSTM1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Mu family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Transferase; Other Amino Acids Metabolism - glutathione; EC 2.5.1.18

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: cytoplasm; cytosol

Molecular Function: enzyme binding; protein homodimerization activity; glutathione binding; glutathione transferase activity

Biological Process: glutathione metabolic process; nitrobenzene metabolic process; xenobiotic metabolic process; xenobiotic catabolic process